Browse > Article
http://dx.doi.org/10.7841/ksbbj.2013.28.5.332

Comparison of Growth Inhibitory Effects on Cancer Cells of Saponin and Fucoidan Treated with Recombinant Thermophilic Xylose Isomerase  

Lee, Dong-Geun (Department of Bioscience and Biotechnology, Graduate School, Silla University)
Park, Seong-Hwan (Department of Bioscience and Biotechnology, Graduate School, Silla University)
Lee, Sang-Hyeon (Department of Bioscience and Biotechnology, Graduate School, Silla University)
Publication Information
KSBB Journal / v.28, no.5, 2013 , pp. 332-337 More about this Journal
Abstract
The gene encoding for xylose isomerase from the thermophilic bacterium Thermotoga maritima was cloned and recombinantly expressed in E. coli cells. Optimal activity was shown at $90^{\circ}C$ and pH 8.0. Treatment of saponin by recombinant xylose isomerase increased the growth inhibitory effect against human gastric cancer (AGS) cells and human colon cancer (HT-29) cells. On the other hand, treatment of fucoidan by the enzyme could not change the growth inhibitory effect against the same cancer cells. One ${\mu}g/ml$ of enzyme-treated saponin exhibited the same or higher growth inhibitory effect against both cancer cells compared with 100 ${\mu}g/ml$ of enzymeuntreated saponin. These results would be useful in the development of functional food or drug.
Keywords
Cancer cells; Fucoidan; Saponin; Thermotoga maritima; Xylose isomerase;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
연도 인용수 순위
1 Elma, Z. T., E. Z. Ilian, and J. H. Christina (1991) Effect of ginsenoside Rg1 on insulin binding in mice liver and brain membrane. Phytother. Res. 5: 46-48.   DOI
2 Kang, S. Y., S. H. Kim, V. B. Schini, and N. D. Kim (1995) Dietary ginsenosides endothlium dependent relaxation in the thoracic arota of hydercholesterolemic rabbit. Gen. Pharmacol. 26: 483-487.   DOI   ScienceOn
3 Lee, K.-S., B.-J. Seong, G.-H. Kim, S.-I. Kim, S.-H. Han, H.-H. Kim, and N.-D. Baik (2010) Ginsenoside, phenolic acid composition and physiological significances of fermented ginseng leaf. J. Kor. Soc. Food Sci. Nutr. 39: 1194-1200.   과학기술학회마을   DOI   ScienceOn
4 Choung, M. G. and E.-H. Sohn. (2011) Anti-tumor activity of saponin fraction of Platycodon gradiflourm through immunomodulatory effects associated with NO production in RAW264.7 cells. Kor. J. Plant Res. 24: 557-563.   과학기술학회마을   DOI   ScienceOn
5 Kim, Y.-C., C.-W. Cho, Y.-K. Rhee, K. M. Yoo, and J. Rho (2007) Antioxidant activity of ginseng extracts prepared by enzyme and heat treatment. J. Kor. Soc. Food Sci. Nutr. 36: 1482-1485.   과학기술학회마을   DOI   ScienceOn
6 Quan, L.-H., Z. Liang, H.-B. Kim, S.-H. Kim, S.-Y. Kim, Y.-D. Noh, and D.-C. Yang (2008) Conversion of ginsenoside Rd to compound K by crude enzymes extracted from Lactobacillus brevis LH8. J. Ginseng Res. 32: 226-231.   과학기술학회마을   DOI   ScienceOn
7 Bioproject, Thermotoga maritima MSB8. http://www.ncbi.nlm.nih.gov/bioproject/111 (2003).
8 Madigan, M. T., J. M. Martinko, and J. Parker (2013) Biology of microorganisms. 10th ed., p. 441. Prentice Hall. London, UK.
9 Walfridsson, M., X. Bao, M. Anderlund, G. Lilius, L. Bulow, and B. Hahn-Hagerdal (1996) Ethanolic fermentation of xylose with Saccharomyces cerevisiae harboring the Thermus thermophilus xylA gene, which expresses an active xylose (glucose) isomerase. Appl. Environ. Microbiol. 62: 4648-4651.
10 Michael, C. A., A. S. Dominic, and M. Anue (1988) Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Cancer Res. 48: 589-595.
11 Nelson, K. E., R. A. Clayton, S. R. Gill, M. L. Gwinn, R. J. Dodson, D. H. Haft, E. K. Hickey, J. D. Peterson, W. C. Nelson, K. A. Ketchum, L. McDonald, T. R. Utterback, J. A. Malek, K. D. Linher, M. M. Garrett, A. M. Stewart, M. D. Cotton, M. S. Pratt, C. A. Phillips, D. Richardson, J. Heidelberg, G. G. Sutton, R. D. Fleischmann, J. A. Eisen, O. White, S. L. Salzberg, H. O. Smith, J. C. Venter, and C. M. Fraser (1999) Genome sequence of Thermotoga maritima: evidence for lateral gene transfer between Archaea and bacteria. Nature 399: 323-329.   DOI   ScienceOn
12 Babich, L., A. F. Hartog, L. J. van Hemert, F. P. Rutjes, and R. Wever (2012) Synthesis of carbohydrates in a continuous flow reactor by immobilized phosphatase and aldolase. ChemSusChem 5: 2348-2353.   DOI   ScienceOn
13 Xu, J., Y. S. Tian, R. H. Peng, B. Zhu, J. J. Gao, and Q. H. Yao (2012) Characterization of a thermostable $\beta$-glucuronidase from Thermotoga maritima expressed in Arabidopsis thaliana. Appl. Microbiol. Biotechnol. 95:1211-1219.   DOI   ScienceOn
14 Rodrigues, M. V., N. Borges, C. P. Almeida, P. Lamosa, and H. Santos (2009) A unique beta-1,2-mannosyltransferase of Thermotoga maritima that uses di-myo-inositol phosphate as the mannosyl acceptor. J. Bacteriol. 191: 6105-6115.   DOI   ScienceOn
15 An. I.-J., S.-D. Cho, J.-K. Kwon, H.-R. Kim, H.-J. Yu, and J.-Y. Jung (2012) The effects of fucoidan on the activation of macrophage and anticancer in gastric cancer cell. J. Fd Hyg. Safety 27: 406-414.   과학기술학회마을   DOI   ScienceOn
16 Schaeffer, D. J. and V. S. Krylov (2000) Anti-HIV activity of extracts and compounds from algae and cyanobacteria. Ecotoxicol. Environ. Saf. 45: 208-227.   DOI   ScienceOn
17 Dobashi, K., T. Nishino, M. Fujihara, and T. Nagumo (1989) Isolation and preliminary characterization of fucose-containing sulfated polysaccharides with blood -anticoagulant activity from the brown seaweed Hizikia fusiforme. Carbohydr. Res. 194: 315-320.   DOI   ScienceOn
18 Hyun, J. H., S. C. Kim, J. I. Kang, M. K. Kim, H. J. Boo, J. M. Kwon, Y. S. Koh, J. W. Hyun, D. B. Park, E. S. Yoo, and H. K. Kang (2009) Apoptosis inducing activity of fucoidan in HCT-15 colon carcinoma cells. Biol. Pharm. Bull. 32: 1760-1764.   DOI   ScienceOn
19 Ko, E. J. and H. G. Joo (2009) Immunostimulatory effects of fucoidan on mouse splenocytes. Lab. Anim. Res. 25: 195-200.   과학기술학회마을
20 Zhou, W., M. Q. Feng, J. Y. Li, and P. Zhou (2006) Studies on the preparation, crystal structure and bioactivity of ginsenoside compound K. J. Asian Nat. Prod. Res. 8: 519-527.   DOI   ScienceOn
21 Jokela, J., O. Pastinen, and M. Leisola (2002) Isomerization of pentose and hexose sugars by an enzyme reactor packed with cross-linked xylose isomerase crystals. Enzyme Microb. Technol. 31: 67-76.
22 Lozada-Ramrez, J. D., A. Snchez-Ferrer, and F. Garca-Carmona (2013) Recombinant S-adenosylhomocysteine hydrolase from Thermotoga maritima: cloning, overexpression, characterization, and thermal purification studies. Appl. Biochem. Biotechnol. 170: 639-653.   DOI   ScienceOn
23 Lee, H. H. and S. T. Jung (2013) Overexpression, crystallization and preliminary X-ray crystallographic analysis of $\beta$-N-acetylglucosaminidase from Thermotoga maritima encoded by the Tm0809 gene. Acta Crystallogr. Sect. F. Struct. Biol. Cryst Commun. 1: 115-117.
24 Ma, Y. H., D. Q. Lv, S. Zhou, D. Y. Lai, and Z. M. Chen (2013) Characterization of an aldo-keto reductase from Thermotoga maritima with high thermostability and a broad substrate spectrum. Biotechnol. Lett. 35: 757-762.   DOI   ScienceOn
25 Chandrakant, P. and V. S. Bisaria (2000) Application of a compatible xylose isomerase in simultaneous bioconversion of glucose and xylose to ethanol. Biotechnol. Bioprocess Eng. 5: 32-39.   과학기술학회마을   DOI   ScienceOn
26 Bucke, C. (1983) Glucose transforming enzymes. pp. 93-127. In: W. Fogarty (ed.). Microbial enzymes and biotechnology. Applied Science Publishers, London, UK.
27 Sambrook, J., E. Fritsch, and T. Maniatis (1989) Molecular Cloning: A Laboratory Manual. 2nd ed., pp. 23-38. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA.
28 Vieille, C., J. Hess, P. K. Kelly, and J. G. Zeikus (1995) XylA cloning and sequencing and biochemical characterization of xylose isomerase from Thermotoga neapolitana. Appl. Environ. Microbiol. 61: 1867-1875.
29 Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.   DOI   ScienceOn