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http://dx.doi.org/10.7841/ksbbj.2013.28.5.315

Bioethanol Production from Eucheuma spinosum using Various Yeasts  

Kim, Min-Ji (Department of Biotechnology, Pukyong National University)
Kim, Jung-Soo (Department of Biotechnology, Pukyong National University)
Ra, Chae Hun (Department of Biotechnology, Pukyong National University)
Kim, Sung-Koo (Department of Biotechnology, Pukyong National University)
Publication Information
KSBB Journal / v.28, no.5, 2013 , pp. 315-318 More about this Journal
Abstract
Ethanol fermentations were performed using separate hydrolysis and fermentation (SHF) processes with monosaccharides from pretreated seaweed, Eucheuma spinosum as the biomass. The pretreatment was carried out with 11% (w/v) seaweed slurry and 150 mM $H_2SO_4$ at $121^{\circ}C$ for 40 min. Enzyme hydrolysis after $H_2SO_4$ pretreatment was performed with Celluclast 1.5 L at $45^{\circ}C$ for 24 h. Five % active charcoal were added to hydrolysate to removed 5-hydroxy methylfurfural. Ethanol fermentation with 11% (w/v) seaweed hydrolysate was performed for 72~96 h using Kluyvermyces marxianus, Pichia stipits, Saccharomyces cervisiae and Candida tropicalis. Ethanol concentration was reached to 18 g/L by K. marxianus, 16 g/L by P. stipitis, 15 g/L by S. cerevisiae and 10 g/L by C. tropicalis, respectively. The ethanol yield from total monosugar was obtained 0.50 and ethanol productivity was obtained 0.38 g/L/h by K. marxianus.
Keywords
Seaweed; Eucheuma spinosum; Ethanol fermentation; Kluyveromyces marxinaus;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 Cho, Y. K., H. J. Kim, and S. K. Kim (2013) Bioethanol production from brown seaweed, Undaria pinnatifida, using NaCl acclimated yeast. Bioprocess. Biosyst. Eng. 36: 713-719.   DOI   ScienceOn
2 Amaya-Delgado, L., E. J. Herrera-Lopez, J. Arrizon, M. Arellano-Plaza, A. Gschaedler (2013) Performance evaluation of Pichia kluyveri, Kluyveromyces marxianus and Saccharomyces cerevisiae in industrial tequila fermentation. World J. Microbiol. Biotechnol. 29: 875-881.   DOI   ScienceOn
3 Fonseca, G. G., E. Heinzle, C. Wittmann, and A. K. Gombert (2008) The yeast Kluyveromyces marxianus and its biotechnological potential. Appl. Microbiol. Biotechnol. 79: 339-354.   DOI
4 Pang, Z. W., J. J. Liang, X. J. Qin, J.R. Wang, J. X. Feng, and R. B. Huang (2010) Multiple induced mutagenesis for improvement of ethanol production by Kluyveromyces marxianus. Biotechnol. Lett. 32: 1847-1851.   DOI
5 Lee, S. M., I. S. Choi, S. K. Kim, and J. H. Lee (2009) Production of bio-ethanol from brown algae by enzymic hydrolysis. KSBB. 24: 483-488.   과학기술학회마을
6 Do, J. H. (1997) Extraction and purification of agar from Gelidium amansii. Kor J Fish Aquatic Sci. 30: 423-427.   과학기술학회마을
7 Yazdani, S. S. and R. Gonzalez (2007) Anaerobic fermentation of glycerol: A path to economic viability for the biofuels industry. Curr. Opin. Biotech. 18: 213-219   DOI   ScienceOn
8 Cazetta, M. L., M. A. P. C. Celligoi, J. B. Buzato, and I. S. Scarmino (2007) Fermentation of molasses by Zymomonas mobilis: Effects of temperature and sugar concentration on ethanol production. Bioresour. Technol. 98: 2824-2828.   DOI   ScienceOn
9 Won, K. (2010) Optimization of dilute acid pretreatment of barley straw for bioethanol production, M.S. Thesis. Dankuk University, Yongin, Korea.
10 Meinita, M. D. N., J. Y. Kang, G.T. Jeong, H. M. Koo, S. M. Park, and Y. K. Hong (2012) Bioethanol production from the acid hydrolysate of the carrageenophyte Kappaphycus alvarezii (cottonii). J. Appl. Phycol. 24: 857-862.   DOI
11 Duckwowrth, M. and W. Yaphe (1971) Structure of ahar. I. Fraction of a complex mixture of polysaccharides. Carbo. Res. 16: 198-197.
12 Seok, J. H., H. G. Park, S. H. Lee, S. W. Nam, S. J. Jeon, J. H. Kim, and Y. H. Kim (2010) High-level secretory expression of recombinant $\beta$-agarose from Zobellia galactanivorans in Pichia pastoris. Kor. J. Microbiol. Biotechnol. 38: 40-45.   과학기술학회마을
13 Tan, I. S., M. K. Lam, and K. T. Lee (2013) Hydrolysis of macroalgae using heterogeneous catalyst for bioethanol production. Carbohydrate. Polymers. 94: 561-566.   DOI   ScienceOn
14 Kim, Y. N., Y. K. Jeong, M. C. Kim, S. B. Kim, Y. K. Chang, W. J. Chi, S. K. Hong, and C. J. Kim (2012) Isolation and identification of agarose-degrading bacterium, Pseudoalteromonas sp. GNUM 08122. Korean J. Microbiol. Biotechnol. 40: 1-9   과학기술학회마을   DOI   ScienceOn