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http://dx.doi.org/10.7841/ksbbj.2013.28.2.123

Effect of Pine needle Ethanol Extracts on the Inhibitory Activity of Atopic Dermatitis  

Jeong, Da-Hyun (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Kim, Koth-Bong-Woo-Ri (Institute of Fisheries Sciences/Pukyong National University)
Jung, Seul-A (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Kim, Hyun-Jee (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Kang, Bo-Kyeong (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Bark, Si-Woo (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Kim, Tae-Wan (Department of Food and Technology, Andong University)
Ahn, Dong-Hyun (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
Publication Information
KSBB Journal / v.28, no.2, 2013 , pp. 123-130 More about this Journal
Abstract
The aim of this study was to examine inhibitory effects of pine needle ethanol extracts (PNEE) on atopic dermatitis (AD). To determine inflammatory activity PNEE was added to LPS-induced murine peritoneal macrophages for an in-vitro test. In addition, anti-AD test was carried out by spreading PNEE on the dorsal skin of 2,4-dinitrochlorobenzene (DNCB)- induced BALB/c mice. It was confirmed that the nitric oxide (NO) secretion was suppressed when $1{\sim}50{\mu}g/mL$ of PNEE were added to LPS-induced murine peritoneal macrophages. Moreover, levels of TNF-${\alpha}$, IL-6, and IL-$1{\beta}$, were decreased. For the anti-AD test, PNEE alleviated symptoms of the erythema in DNCB-induced mice. Furthermore, the IFN-${\gamma}$ secretion of the group treated with PNEE was increased in splenocytes from DNCB-induced mice compared to the positive control, while IL-4 secretion diminished. Through these results, we can conclude that PNEE can inhibit AD by modulating the IFN-${\gamma}$, IL-4 cytokines production and inhibiting inflammation.
Keywords
Pine needle; Anti-atopy; Anti-inflammation;
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