Browse > Article
http://dx.doi.org/10.7841/ksbbj.2012.27.3.172

Isolation of Arthrospira platensis Mutants Producing High Lipid and Phycobiliproteins  

Kim, Young-Hwa (Department of Pharmaceutical Engineering, Silla University)
Lee, Jae-Hwa (Department of Pharmaceutical Engineering, Silla University)
Publication Information
KSBB Journal / v.27, no.3, 2012 , pp. 172-176 More about this Journal
Abstract
In this study, microalgae Arthrospira platensis (A. platensis) mutants induced by ethyl methane sulfonate (EMS) and further selection for resistance of cerulenin, a potent inhibitor of fatty acid synthase, were characterized. The mutants selected by $2{\mu}M$, $5{\mu}M$ and $10{\mu}M$ of cerulenin were designated EC2, EC5 and EC10, respectively. Under normal growth conditions, the mutants and parental strain exhibited similar growth pattern. The mutants of A. platensis showed enhanced lipid accumulation and phycobiliproteins (phycoerythrin, phycocyanin). The lipid content of mutants EC2 and EC5 was about 4.4 and 4.8-fold higher than wild type. The phycoerythrin and phycocyanin content of mutants EC2 and EC5 was increased about 1.5 and 6.9-fold and 1.4 and 3.8-fold, respectively, compared to the wild type. The chlorophyll and carotenoid content of mutants was slightly increased. The high lipid and pigment contents exhibited by A. platensis mutants would make an excellent candidate for the production of commercially interesting biologically active compounds.
Keywords
microalgae; Arthrospira platensis; mutants; lipid; phycobiliproteins;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Datz, G. and G. Dohler (1981) Light dependent changes in the lipid and fatty acid composition of phycocyanin free photosynthetic lamellae of Synechococcus. Z. Naturforsch. 36: 856-862.
2 Schoefs, B., N. E. Rmiki, J. Rachadi, and Y. Lemoine (2001) Astaxathin accumulation in Haematococcus requires a cytochrome P450 hydroxylase and an active synthesis of fatty acids. FEBS Letters 500: 125-128.   DOI
3 Solovchenko, A. E., I. Khozin-Goldberg, S. Didi-Cohen, Z. Cohen, and M. N. Merzlyak (2008) Effects of light and nitrogen starvation on the content and composition of carotenoids of the green microalga Parietochloreis incisa. Russ. J. Plant Physiol. 53: 455-462.
4 Fischer, R. (1998) Isolation of mutants, a key for the analysis of complex pathways and for strain improvement. In Microbes for Health, Wealth and Sustainable Environment. pp. 739-751. Verma, A., Ed. Malhortra Publishing House, New Delhi, India.
5 Wang, J., R. Li, D. Lu, S. Ma, Y. Yan, and W. Li (2009) A quick isolation method for mutants with high lipid yield in oleaginous yeast. World J. Microbiol. Biotechnol. 25: 921-925.   DOI
6 Chaturvedi, R. and Y. Fujita (2006) Isolation of enhanced eicosapentaenoic acid producing mutants of Nannochloropsis oculata ST-6 using ethyl methane sulfonate induced mutagenesis techniques and their characterization at mRNA transcript level. Phycol. Res. 54: 208-219.   DOI
7 Meireles, L. A., A. C. Guedes, and F. X. Malcata (2003) Increase of the yields of eicosapentaenoic and docosahexaenoic acids by the microalga Pavlova lutheri following random mutagenesis. Biotechnol. Bioeng. 81: 50-55.   DOI
8 Chen, W., M. Sommerfeld, and Q. Hu (2011) Microwave-assisted nile red method for in vivo quantification of neutral lipids in microalgae. Biores. Technol. 102: 135-141.   DOI
9 Shukla, S. P. and A. K. Kashyap (2003) An assessment of biopotential of three cyanobacterial isolates from antarctic for carotenoid production. Indian J. of Biochem. Biophys. 40: 362-366.
10 Joo, D. S. and S. Y. Cho (2000) Stability of phycocyanin and spectral characteristic of phycobilins from Spirulina platensis. J. Kor. Fish. Soc. 33: 482-488.   과학기술학회마을
11 Mata, T. M., A. A. Martins, and N. S. Caetano (2010) Microalgae for biodiesel production and other applications: a review. Renew. Sustain. Energy Reviews. 14: 217-232.   DOI
12 Singh, S. C., R. P. Sinha, and D. P. Häder (2002) Role of lipids and fatty acids in stress tolerance in cyanobacteria. Acta. Protozool. 41: 297-308.
13 Gill, I. and R. Valivaty (2001) Polyunsaturated fatty acid: occurrence, biological applications. Trends Biotechnol. 15: 401-409.
14 Bhat, V. B. and K. M. Madyastha (2000) C-Phycocyanin: a potent peroxyl radical scavenger in vivo and in vitro. Biochem. Biophys. Res. Commun. 275: 20-25.   DOI   ScienceOn
15 Abd, El-Baky H., K. Farouk, and S. Gamal (2007) Production of carotenoids from marine microalgae and its evaluation as safe food colorant and lowering cholesterol agent. American-Eurasian J. of Agricult. Environ. Sci. 2: 792-800.
16 Carvalho, A. P. and F. X. Malcata (2000) Effect of culture media on production of polyunsaturated fatty acids by Pavlova lutheri. Cryptogram Algol. 21: 59-71.   DOI
17 Harun, R., M. Singh, G. M. Forde, and M. K. Danquah (2010) Bioprocess engineering of microalgae to produce a variety of consumer products. Renew. Sustain. Energy Reviews 14: 1037-1047.   DOI
18 Roman, R. B., J. M. Alvarez-PeZ, F. G. A. Fernandez, and E. M. Grima (2002) Recovery of pure B-phycoerythrin from microalgae Porphyridium cruentum. J. Biotechnol. 93: 73-85.   DOI
19 Heath, R. J., S. W. White, and C. O. Rock (2001) Lipid biosynthesis as a target for antibacterial agents. Prog. Lipid Res. 40: 467-497.   DOI
20 Saitoh, S., K. Takahashi, K. Nabeshima, Y. Yamashita, Y. Nakaseko, A. Hirata, and M. Yanagida (1996) Aberrant mitosis in fission yeast mutants defective in fatty acid synthetase and acetyl CoA carboxylase. J. Cell Biol. 134: 949-961.   DOI
21 Gantar, M. and Z. Swircev (2008) Microalgae and cyanobacteria: food for thought. J. of Phycol. 44: 260-268.   DOI
22 Carlton, B. C. and B. J. Brown (1981) Gene mutation. In Gerhardt, P. (Ed.) Manual of Methods for General Bacteriology. pp. 85-96. American Society for Microbiology, Washington, DC, USA.
23 Kim, Y. M., M. R. Kim, T. H. Kwon, J. M. Ha, and J. H. Lee (2009) Optimum culture conditions for the growth of Spirulina platensis NIES 39. J. Kor. Ind. Eng. Chem. 20: 285-289.   과학기술학회마을