Browse > Article
http://dx.doi.org/10.7841/ksbbj.2012.27.1.057

Evaluation of Anti-diabetic Effect of Biochanin A in C2C12 Myotube  

Hwang, Jin-Taek (Korea Food Research Institute)
Kim, Sung-Hee (Korea Food Research Institute)
Publication Information
KSBB Journal / v.27, no.1, 2012 , pp. 57-60 More about this Journal
Abstract
In this study, we evaluated the effects of Biochanin A on glucose uptake in C2C12 myotube. We found that Biochanin A significantly stimulated 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-d-glucose (2-NBDG) uptake in a dose-dependent manner. In addition, AMPK and PPAR-gamma activities were markedly increased by Biochanin A in a dose-dependent manner. However, Akt, an insulin dependent signaling molecule, did not change by Biochanin A. These results suggest that Biochanin A stimulates glucose uptake via AMPK and PPAR-gamma pathways.
Keywords
Biochanin A; Anti-diabetes; C2C12 myotube; PPAR-gamma; AMP-activated protein kinase;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Kole, L., B. Giri, S. K. Manna, B. Pal, and S. Ghosh (2011) Biochanin-A, an isoflavon, showed anti-proliferative and anti-inflammatory activities through the inhibition of iNOS expression, p38-MAPK and ATF-2 phosphorylation and blocking $NF{\kappa}B$ nuclear translocation. Eur. J. Pharmacol. 653: 8-15.   DOI
2 Moon, Y. J., B. S. Shin, G. An, and M. E. Morris (2008) Biochanin A inhibits breast cancer tumor growth in a murine xenograft model. Pharm. Res. 25: 2158-2163.   DOI
3 Meinwald, J. (2011) Natural products as molecular messengers. J. Nat. Prod. 74: 305-309.   DOI
4 Genovese, S. and F. Epifano (2011) Auraptene: a natural biologically active compound with multiple targets. Curr. Drug Targets 12: 381-386.   DOI
5 Dolinsky, V. W. and J. R. Dyck (2011) Calorie restriction and resveratrol in cardiovascular health and disease. Biochim. Biophys. Acta. 1812: 1477-1489.   DOI
6 Lee, M. S., C. H. Kim, D. M. Hoang, B. Y. Kim, C. B. sohn, M. R. Kim, and J. S. Ahn (2009) Genistein-derivatives from tetracera scandens stimulate glucose-uptake in L6 myotubes. Biol. Pharm. Bull. 32: 504-508.   DOI
7 Cartee, G. D. and J. F. Wojtaszewski (2007) Role of Akt substrate of 160 kDa in insulin-stimulated and contraction-stimulated glucose transport. Appl. Physiol. Nutr. Metab. 32: 557-566.   DOI   ScienceOn
8 Cho, N. and Y. Momose (2008) Peroxisome proliferator-activated receptor gamma agonists as insulin sensitizers: from the discovery to recent progress. Curr. Top Med. Chem. 8: 1483-1507.   DOI
9 Zou, C., Y. Wang, and Z. Shen (2005) 2-NBDG as a fluorescent indicator for direct glucose uptake measurement. J. Biochem. Biophys. Methods 64: 207-215.   DOI
10 Lee, M. S., J. T. Hwang, S. H. Kim, S. Yoon, M. S. Kim, H. J. Yang, and D. Y. Kwon (2010) Ginsenoside Rc, an active component of Panax ginseng, stimulates glucose uptake in C2C12 myotubes through an AMPK-dependent mechanism. J. Ethnopharmacol. 127: 771-776.   DOI
11 Hardie, D. G. (2011) Sensing of energy and nutrients by AMP-activated protein kinase. Am. J. Clin. Nutr. 93: 891S-896S.   DOI
12 Stolarczyk, M., W. Gutman, and R.A. Derlacz (2011) Nuclear receptors PPAR as a drug target in metabolic disorders. Postepy Biochem. 57:207-14.
13 Wong, A. K., J. Howie, J. R. Petrie, and C. C. Lang (2009) AMP-activated protein kinase pathway: a potential therapeutic target in cardiometabolic disease. Clin. Sci (Lond). 116: 607-620.   DOI