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http://dx.doi.org/10.7841/ksbbj.2012.27.1.033

Identification of Curcuminoids from Turmeric (Curcuma longa) Using Ultrasonic Wave and Dipping Method  

Lee, Kwang-Jin (TKM-Based Herbal Drug Research Group, KIOM)
Ma, Jin-Yeul (TKM-Based Herbal Drug Research Group, KIOM)
Kim, Young-Sik (Department of Chemical Engineering, Kangwon National University)
Publication Information
KSBB Journal / v.27, no.1, 2012 , pp. 33-39 More about this Journal
Abstract
In this study three major curcuminoids in turmeric curcumin (1), demethoxycurcumin (2) and bismethoxycurcumin (3) were efficiently extracted by optimizing extraction condition and simultaneously identified using a fast and reliable RP-HPLC-UV-MS and TLC method. The analysis by the $C_{18}$ column was performed and the UV wavelength was fixed at 425 nm. In this result, the total extraction yield of turmeric (Curcuma longa) was increased with extraction time from 1 to 7 h. So, optimum extraction time is 4 h. Also, the highest yield of extraction amount 0.433g 8.66% was obtained by ultrasonic waves with quarter frequency kHz and an extraction time of 7 h. The experiment method was consistent with theoretical Value $r^2$ = 0.987 (1), 0.997 (2) and 0.998 (3). Moreover, LC-MS analysis provided efficiently molecular weight information of three major curcuminoids in turmeric extracts and high purity (~95%) of the curcuminoids were obtained. This work offers would be useful for chemical and biological studies of natural plants and its products.
Keywords
Analysis; Curcuma longa; Curcuminoids; HPLC; Separation; Isolation;
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