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http://dx.doi.org/10.7841/ksbbj.2011.26.3.206

Effect of Alginate on the Growth of Nannochloropsis oculata NIES-2145  

Park, Hyun-Jin (Department of Bioscience and Biotechnology, College of Medical and Life Science, Silla University)
Kim, Young-Hwa (Department of Pharmaceutical Engineering, College of Medical and Life Science, Silla University)
Lee, Jae-Hwa (Department of Bioscience and Biotechnology, College of Medical and Life Science, Silla University)
Publication Information
KSBB Journal / v.26, no.3, 2011 , pp. 206-210 More about this Journal
Abstract
The growth effect of Nannochloropsis oculata (N.oculata), unicellular microalgae, by alginate was investigated. Alginate was depolymerized with sulfuric acid ($H_2SO_4$) and heat ($121^{\circ}C$), simultaneously. Addition of 0.75% alginate oligomer depolymerized with 0.2 N $H_2SO_4$ showed the maximum yield and the growth rate of N. oculata. Chlorophyll content and reducing sugar was increased by alginate oligomer in a dose-dependent manner. Alginate oligomer promoted the growth of N. oculata, whereas the original alginate polysaccharides had no significant effect. Laminaria japonica (L. japonica) extract containing high level of alginate was also increased growth rate and chlorophyll content. $CO_2$ supply addition to L. japonica extract showed no change the growth rate, although addition to alginate oligomer showed prominently increased. N. oculata could use more saccharides in presence of $CO_2$ according to reducing sugar determination. From these results, it is useful to establish optimal condition for high cell density cultivation of N. oculata.
Keywords
Alginate; Nannochloropsis oculata; Laminaria japonica; $CO_2$;
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