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http://dx.doi.org/10.7841/ksbbj.2011.26.1.019

Isolation and Antibacterial Activity of Actinomycetes Producing Growth Inhibition Compounds Against Multi-antibiotic Resistant Pseudomonas Aeruginosa  

Kang, Dong-Hee (Department of Microbiology, College of Natural Science, Keimyung University)
Bae, Ho-Kyung (Department of Microbiology, College of Natural Science, Keimyung University)
Kim, Hyun-Soo (Department of Microbiology, College of Natural Science, Keimyung University)
Publication Information
KSBB Journal / v.26, no.1, 2011 , pp. 19-26 More about this Journal
Abstract
Of the 500 Actinomycetes isolates obtained from soil, one isolate grown on maltose as the sole carbon source produced compound BHK-P19, which inhibited the growth of multiple drug resistant P. aeruginosa 0245. Ultraviolet radiation mutagenesis curtailed production of BHK-P19. Mutation of the BHK-P19 producer using N-methyl-N'-nitro-N-nitroso-guanidine obviated the antibacterial activity to P. aeruginosa 0245, but not towards P. aeruginosa 0225. The mixing of BHK-P19 and BHK-S5 culture extracts inhibited P. aeruginosa 0254, 0225 and 1113. The combined application of BHK-P19 culture extract and Schizandra chinensis Baillon extract inhibited P. aeruginosa 0254, 0225, 0826, 1113, 1378, 1731 and 2492. Use of various concentrations of BHK-P19 culture extract and ampicillin markedly increased antibacterial activity against multi-drug resistant P. aeruginose 1113.
Keywords
multi-antibiotic resistance; pseudomonas aeruginosa; antibiotics; actinomycetes;
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