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Prediction of ORFs in Metagenome by Using Cis-acting Transcriptional and Translational Factors  

Cheong, Dea-Eun (Department of Biological Sciences, College of Natural Sciences, Chonnam National University)
Kim, Geun-Joong (Department of Biological Sciences, College of Natural Sciences, Chonnam National University)
Publication Information
KSBB Journal / v.25, no.5, 2010 , pp. 490-496 More about this Journal
Abstract
As sequencing technologies are steadily improving, massive sequence data have been accumulated in public databases. Thereby, programs based on various algorithms are developed to mine useful information, such as genes, operons and regulatory factors,from these sequences. However, despite its usefulness in a wide range of applications, comprehensive analyses of metagenome using these programs have some drawbacks, thereby yielding inaccurate or complex results. We here provide a possibility of signature sequences (cis-acting transcriptional and translational factors of metagenome) as a hallmark of ORFs finding from metagenome.
Keywords
Metagenome; annotation; ORF prediction; promoter; RBS;
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