Browse > Article

Development of a Microbial Biosurfactant Production Process  

Kim, Ju-Hyun (Department of Biotechnology, Seokyeong University)
Kim, Gi-Eun (Department of Biotechnology, Seokyeong University)
Publication Information
KSBB Journal / v.24, no.2, 2009 , pp. 140-148 More about this Journal
Abstract
The microbial biosurfactants can be substituted to the chemical detergents in some industrial processes. In this study we developed a biotechnological processes for the biosurfactants with microorganisms. The biosurfactants have a lot of advantages in comparision with the chemical surfactants. They are proenvironmental even during and after industrial use. But there are not so many kinds of biosurfactants. The production cost and the end price is much higher than the chemical surfactants. But nowdays there are many kinds of microorganisms, which can produce the surfactants in large quantity and fast. We tried to develop a production process for the large scale with some microorganisms. At first Candida bombicola KCTC 7145, Sphingomonas chungbukensis KCTC 2955 and Sphingomonas yanoikuyae KCTC 2818 are cultivated and studied. For the large scale production process we used molasses as a complex medium and tried to optimize the process. Molasses contains 17 to 25% of water, 45 to 50% of sugar and 25% of carbohydrate, it can be fully used as a substrate. The microorganisms have been cultivated in the diluted media with molasses 2, 5, 8 and 10%, respectively, The optimal conditions for the cultivation and the production process have been studied. For the study the optical density, glucose concentration and the surface tension were measured. Candida bombicola KCTC 7145 and the 5% molasses media was selected as an optimal condition for the production process of a biosurfactant. During cultivation of Candida bombicola KCTC 7145 in the 5% molasses medium kerosene and corn oil were added for promoting the biosurfactants.
Keywords
Biosurfactant; Molasses; Candida bombicola KCTC 7145; Surface tension; Kerosene; Corn oil;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Van Dyke, M. I., P. Couture, M. Brauer, H. Lee, and J. T. Trevors (1993), Pseudomonas aeruginosa UG2 rhanmolipid biosurfactants : structural chracterization and their use in removing hydrophobic compounds from soil. Can J. Microbial. 39(11), 1071-1078   DOI   ScienceOn
2 Robert, M., M. E. Mercade, M. P. Bosch, J. L. Parra, M. J. Espuny, M. A. Manresa, and J. Guinea (1989), Effect of the carbon source on biosurfactant production by Pseudomonas aeruginosa 44T. Biotechnol, Lett. 11, 871-874   DOI
3 Desai, J. D. and I. M. Banet (1997), Microbial production of surfactants and their commercial potentiaI, Microbiol. Mol. Biol. Rev. 61(1), 47-64   PUBMED
4 Milton, M. J. (1987), Surfactants in engineering technology, Surfactant Science Series 26, Marcell Dekker, New York
5 Dunvnjak, J. D., G. Cooper, and N. Kosaric (1982), Production of biosurfactant by Arthrobacter paraffineus ATCC 19558, Biotechnol. Bioeng. 24, 165-175   DOI   PUBMED
6 Rosen. M. J. (1989), in Surfactant and Interfacial Phenomena, 2nd ed., Wiley. New york
7 Neu. T. R. and K. Poralla (1990), Emulsifying agent from bacteria isolated during screening for cells with hydrophobic surfaces. Appl. Microbiol. Biotechnol. 32, 521-525   DOI
8 Cooper, D. G., J. E. Zajic, and D. F. Gerson (1978), Production of surface active lipids by Corynebacterium lepus. Appl. Environ. Microbiol. 37, 4-10
9 Bognolo, G. (1999), Biosurfactants as emulsifying agents for hydrocarbons. Colloids surfaces A: Physicochem Eng Aspects 152, 41-52   DOI   ScienceOn
10 Massaki, M., D. Hiromi, T. Toshifumi, M. Satoru, S. Yasutsugu, and I. Tadayuki (1993), A new lipopeptide biosurfactant produced by Arthrobacter sp. Strain MIS38. J. Bacteriol. 175, 6459-6466   DOI   PUBMED
11 Makkar, R. S. and S. C. Cameotra, (1997b), Utilization of molasses for biosurfactant production by two Bacillus strains at thermophilic conditions. J. Am. Oil. Chem. Soc. 74(7), 887-889   DOI   ScienceOn
12 Arima, K., A. Kakinuma, and G. Tamura (1968), Surfactin, a crystalline peptide lipid surfactant produced by Bacillus subtilis : isolation, characterization and its inhibition of fibrin clot formation. Biochem. Biophys. Res. Commun. 31, 488-494   DOI   ScienceOn
13 Cooper, D. G., S. N. Liss, R. Longay, and J. E. Zajic (1989), Surface activities of Mycobacterium and Pseudomonas. J. Ferment. Technol. 59, 97-101
14 Kim, S. H., E. J. Lim, K. S. Choi, Y. K. Jeong, K. L. Jang, and T. H. Lee (1996), Emulsifying agent production by Acinetobacter sp. BE-254, Kor. J. Appl. Microbial. Biotechnol. 24, 206-212
15 Banat, I. M., R. S. Markkar, and S. S. Cometra (2000), Potential commercial appIications of microbial surfactants, Appl. Micribial. Biotechnol. 53, 495-508   DOI   ScienceOn
16 Javaheri, M., G. E. Jenneman, M. J. McInnemey, and R. M. Knapp (1985), Anaerobic production of a biosurfactant by Bacillus licheniformis JF-2. Appl. Environ. Microbiol. 50, 698-700   PUBMED
17 Kosaric, N., C. C. Gray, and W. L. Carin (1983), Microbial emulsifiers and deemulsifiers. Biotechnology 3, 575-592
18 Fiechter, A. (1992), Integrated systems for biosurfactant synthesis. Pure Appl. Chem. 64(11), 1739-1743   DOI
19 Poremba, K., W. Gunkel, S. Lang, and F. Wagner (1991), Marine biosurfactants, III. toxicity testing with marine microorganism and comparison with synthetic surfactants, Z. Naturforch 46c, 21-216, 14
20 Najafpour, G. D. and C. P. Shan (2003), Enzymatic hydrolysis of molasses. Bioresour. Technol. 86, 91-94   DOI   ScienceOn
21 Yakimov, M. M., K. N. Timmis, V. Wray, and H. L. Fredrickson (1995), Characterization of a new lipopeptide surfactant produced by thermotolerant and halotolerant subsurface Bacillus licheniformis BAS50. Appl. Environ. Microbiol. 61, 1706-1713   PUBMED
22 Shuler, M. L., F. Karg (2003), Bioprocess engineering, Prentice Hall. 194-196
23 Cooper, D. G. and D. A. Paddock (1984), ' Production of a biosurfactant from Torulopsis bombicola', Appl. Environ. Microbiol. 47, 173-176   PUBMED   ScienceOn
24 Magaritis, A., A. Kennedy, J. E. Zajic, and D. F. Gerson (1979), Biosurfactant production by Nocardia erythropolis. Dev. lnd. Microbiol. 20, 623-630
25 Desai, A. J., K. M. Patal, and J. D. Desai (1988), Emulsifier production by Pseudomonas fluorescens during the growth on hydrocarbons. Curr. Sci. 57, 500-501
26 Zajic, J. E., H. Gignard, and D. F. Gerson (1977), Properties and biodegradation of a bioemulsifier from Corynebacterium hydrocarboclastus, Biotechnol. Bioeng. 19, 1303-1320   DOI   ScienceOn
27 Lang, S. (2002), Biological amphiphiles (microbial biosurfactants). Current Opinion in colloid & Interface Science. 7, 12-20   DOI   ScienceOn
28 Bernheimer, A. W. and L. S. Avigad (1970), Nature and properties of a cytological agent produced by Bacillus subtilis. J. Gen. Microbiol. 61, 361-369   DOI   PUBMED   ScienceOn
29 Um, H. J., M. H. Kang, Y. P. Kim, S. J. Kim, and Y. C. Kim (2003), Sequence and phylogenetic analysis of the phnS gene encoding 2-hydroxy chromene-2- carboxylate isomerase in Sphingomonas chungbukensis DJ77. The Korean Journal of Microbiology 39(3), 123-127
30 Persson, A., E. Oesterberg, and M. Dostalek (1988), Biosurfactant production by Pseudomonas Fluorescens 378: Growth and product ch따acteristics. Appl. Microbiol. Biotechnol. 29, 1-4   DOI
31 Layman, P. (1985), Industrial set for strong growth. Chem. Eng. News 63, 23
32 Beersteacher, E. (1954), Petroleum Microbiology. Elsevier. Houston
33 Guεrra-Santos , L. H., O. Kappeli, and A. Flechter (1986), Dependence of Pseudomonas aeruginosa continuous culture biosurfactant production on nutritional and environmental factors. Appl. Microbiol. Biotechnol. 24, 443-448   DOI
34 MacFaddin. J. F. (1984), Biochemical Tests for Identification for Medical Bacterial, 2nd ed. Williams and Wilkins Co., Baltimore, USA
35 Schippers, C., K. Gessner, T. Muller, and T. Scheper (2000), Microbial degradation of phenanthrene by addition of a sophorelipid mixture. J. Biotechnol. 83(3), 189-198   DOI   ScienceOn
36 Asmer, H., S. Lang., F. Wagner, and V. Wray (1988), Microbial production, structureelucidation and bioconversion of sophorose lipids. J. Am Oil Chem Soc. 65, 1460-1466   DOI
37 Lim, K. H. (1996), Their structure, properties, and applications, J. Korean Oil Chemist's Soc. 13, 1-20
38 Ito, A., S. Inoue (1982), Sopholipids from Toulopsis bombicola; possible relation to alkane uptake. Appl Environ Microbial. 43, 1278-1283