Browse > Article

Utilization of pollen grains for the expression of epidermal growth factor  

Choi, Byung-Jin (Department of Horticulture, Department of Biotechnology, Catholic University of Daegu Kyungsan)
Park, Hee-Sung (Department of Horticulture, Department of Biotechnology, Catholic University of Daegu Kyungsan)
Publication Information
KSBB Journal / v.23, no.5, 2008 , pp. 460-462 More about this Journal
Abstract
Pollens grains collected from fully dehisced lily (Lilium longiflorum) anthers were given wounds by means of shaking in the presence of aluminum oxide particles. They were transformed by infiltration with Agrobacterium cells harboring a synthetic DNA encoding signal peptide-fused epidermal growth factor (EGF). After incubation for 24 hr in vitro, the pollen culture showed that EGF mRNAs and proteins were successfully expressed in the analysis of cDNA blot hybridization and immuno-blotting.
Keywords
lily pollen; agroinfiltration; epidermal growth factor; immuno-blotting;
Citations & Related Records
연도 인용수 순위
  • Reference
1 McCormick, S. (1993), Male gametophyte development, Plant Cell 5, 1265-1273   DOI   ScienceOn
2 Taylor, L. P. and P. K. Helper (1997), Pollen germination and tube growth, Annu. Rev. Plant Physiol. Plant Mol. Biol. 48, 461-491   DOI
3 Aronen, T. S., T. O. Nikkanen, and H. M. Haggman (1998), Compatability of different pollination techniques with microprojectile bombardment of Norway spruce and Scots pine pollen, Can. J. For. Res. 28, 79-86   DOI
4 Fernando, D. D., J. N. Owens, and S. Misra (2000), Transient gene expression in pine pollen tubes following particle bombardment, Plant Cell Rep. 19, 224-228   DOI
5 Hess, D., K. Dressler, and R. Nimmrichter (1990), Transformation experiments by pipetting Agrobacterium into the spikelete of wheat (Triticum aestivum L.), Plant Sci. 72, 233-244   DOI   ScienceOn
6 Luo, Z. X. and R. Wu (1989), A simple method for the transformation of rice via the pollen-tube pathway, Plant Mol. Biol. Rep. 7, 69-77   DOI
7 Tjokrokusumo, D., T. Heinrich, S. Wylie, R. Potter, and J. McComb (2000), Vacuum infiltration of Petunia hybrida pollen with Agrobacterium tumefaciens to achieve plant transformation, Plant Cell Rep. 19, 792-797   DOI
8 Gnanasambandam, A., I. G. Polkinghorne, and R. G. Birch (2007), Heterologous signals allow efficient targeting of a nuclear-encoded fusion protein to plastids and endoplasmic reticulum in diverse plant species, Plant Biotech. J. 5, 290-296   DOI   ScienceOn
9 Kim, S. S., D. I. Shin, and H. S. Park (2007), Transient ${\beta}$- glucuronidase expression in lily pollen via wounding-assisted Agrobacterium-mediated transformation, Biotech. Lett. 29, 965-969   DOI
10 Bell, G. I., N. M. Fong, M. M. Stempien, M. A. Wormsted, D. Caput, L. Ku, M. S. Urdea, L. B. Rall, and R. Sanchez-Pescador (1986), Human epidermal growth factor precursor: cDNA sequence, expression in vitro and gene organization, Nucl. Acid. Res. l, 8427-8446
11 Langridge P., R. Brettschneider, P. Lazzeri, and H. Lorz (1992), Transformation of cereals via Agrobacterium and the pollen pathway; a critical assessment, Plant J. 2, 631-638   DOI
12 Hollenber, M. D. and H. Gregory (1997), Human urogastrone and mouse epidermal growth factor share a common receptor site in cultured human fibroblasts, Life Sci. 20, 267-74