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The ceramide contents of Saccharomyces cerevisiae in batch culture  

Kim, Se-Kyung (Department of Biological Engineering, Inha University)
Noh, Yong-Ho (Department of Biological Engineering, Inha University)
Yun, Hyun-Shik (Department of Biological Engineering, Inha University)
Publication Information
KSBB Journal / v.23, no.5, 2008 , pp. 449-451 More about this Journal
Abstract
Ceramide has become a widely used ingredient in cosmetic and pharmaceutical industries, however, only a few yeast strains were investigated for the synthesis of ceramide and the concentration was very low. Ceramide is not only a core intermediate of sphingolipids but also an important modulator of many cellular events including apoptosis, cell cycle arrest, senescence, differentiation, and stress responses. In this study S.cerevisiae was grown in a batch culture and the cellular content of ceramide was measured at different growth phases. The ceramide content was highest at stationary phase and 2.01 mg ceramide/g cell was obtained.
Keywords
Saccharomyces cerevisiae; ceramide; growth phases;
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Times Cited By KSCI : 1  (Citation Analysis)
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1 Rupeic, J., M. Maaric, and V. Maric (1998), The influence of carbon source on the level and composition of ceramides of the Candida lipolytica yeast, Appl. Microbiol. Biotechnol. 50, 583-588   DOI
2 Cuvillier, O. (2002), Sphingosine in apoptosis signaling, Biochim. Biophys. Acta 1585(2-3), 153-162   DOI   ScienceOn
3 Hannum, Y. A. and Bell R. M. (1987), Lysosphingolipid inhibit protein kinase C: implications for the sphingolipidoses. Science 235(4789), 670-674   DOI
4 Im, D. S. (2004), Sphingosine, Sphingosine kinase and Apoptosis, Biochem. Mol. Biol. News 24(1), 38-72
5 Dickson, R. C. and R. L. Lester (1999), Yeast sphingolipid, Biochim. Biophys. Acta 1426, 347-357   DOI   ScienceOn
6 Thomas, J. M., E. C. Aida, R. B. Phyllis, and S. F. Anthony (1999), The separation and direction detection of ceramide and sphingoid bases by normal-phase high performance liquid chromatography and evaporative light-scattering detection, Anal. Biochem. 276, 242-250   DOI   ScienceOn
7 Lynch, K. R. and D. S. Im (1999), Life on the edg, Trends pharmacol. Sci. 20(12), 473-475   DOI   ScienceOn
8 Hong, S. P., C. H. Lee, S. K. Kim, H. S. Yun, J. H. Lee, and K. H. Row (2004), Mobile phase compositions for ceramide III by normal phase high performance liquid chromatography, Biotechnol. Bioprocess Eng. 9, 47-51   DOI   ScienceOn
9 Rupeic, J. and V. Maric (1998), Isolation and chemical composition of the ceramide of the Candida lipolytica yeast, Chem. Phys. Lipids 91, 153-161   DOI   ScienceOn