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Development of Magnetically Separable Immobilized Trypsin  

Ryu, Ji-Soon (Department of Chemical and Biochemical Engineering, Chosun University)
Lee, Jung-Heon (Department of Chemical and Biochemical Engineering, Chosun University)
Publication Information
KSBB Journal / v.23, no.4, 2008 , pp. 350-354 More about this Journal
Abstract
Magnetically separable immobilized trypsin was developed and their biocatalytic activity was evaluated for the different immobilization media. The activity, recyclability, pH effect, and stability of immobilized enzymes were evaluated for the different supporting media. The biocatalytic activity of immobilized trypsin was highest with magnetically separable polyaniline (PAMP), and Vm and Km of PAMP were 0.169 mM/min and 0.263 mM respectively. With increasedpH, the biocatalytic activity increased for all supporting materials used. Immobilized enzymes were recycled and recycle activities were over 90% of their original activity after ten times reuse. The operational stabilities of enzymes were greatly improved with enzyme immobilization.
Keywords
Trypsin; Magnetically separable; immobilized enzyme;
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1 Stults, J. T., Arnott, D., and Burlingame, A. L. (2005), Proteomics. In Methods in Enzymology. p245, Academic Press
2 Kong, X., Zhou, H., and Qian, H. (2007), Enzymatic preparation and functional properties of wheat gluten hydrolysates. Food Chemistry 101, 615-620   DOI   ScienceOn
3 Ringseis, R., Matthes, B., Lehmann, V., Becker, K., Schops, R., Ulbrich-Hofmann, R., and Eder, K. (2005), Peptides and hydrolysates from casein and soy protein modulate the release of vasoactive substances from human aortic endothelial cells. Biochimica et Biophysica Acta (BBA)-General Subjects, 1721, 89-97   DOI   ScienceOn
4 Kim, J., Lee, J., Na, H. B., Kim, B. C., Youn, J. K., Kwak, J. H., Moon, K., Lee, E., Kim, J., Park, J., Dohnalkova, A., Park, H. G., Gu, M. B., Chang, H. N., Grate, J. W., and Hyeon, T. (2005), A magnetically separable, highly stable enzyme system based on nanocomposites of enzymes and magnetic nanoparticles shipped in hierarchically ordered, mesocellular, mesoporous silica. Small 1, 1203-1207   DOI   ScienceOn
5 Simonian, A. L., Good, T. A., Wang, S. S., and Wild, J. R. (2005), Nanoparticle-based optical biosensors for the direct detection of organophosphate chemical warfare agents and pesticides. Analytica Chimica Acta 534, 69-77   DOI   ScienceOn
6 Ota, S., Miyazaki, S., Matsuoka, H., Morisato, K., Shintani, Y., and Nakanishi, K. (2007), High-throughput protein digestion by trypsin-immobilized monolithic silica with pipette-tip formula. Journal of Biochemical and Biophysical Methods 70, 57-62   DOI   ScienceOn
7 Barrett, J., Brophy, P. M., and Hamilton, J. V. (2005), Analysing proteomic data. International Journal for Parasitology 35, 543   DOI   ScienceOn
8 Lee, J., Kim, J., Kim, J., Jia, H. F., Kim, M. I., Kwak, J. H., Jin, S. M., Dohnalkova, A., Park, H. G., Chang, H. N., Wang, P., Grate, J. W., and Hyeon, T. (2005), Simple synthesis of hierarchically ordered mesocellular mesoporous silica materials hosting crosslinked enzyme aggregates. Small 1, 744-753
9 Mitsui, K., Doi, H., Nukina, N., and Indu Kheterpal and Ronald, W. (2006) Proteomics of Polyglutamine Aggregates. In Methods in Enzymology pp63-76, Academic Press
10 Gibbs, B. F., Zougman, A., Masse, R., and Mulligan, C. (2004), Production and characterization of bioactive peptides from soy hydrolysate and soy-fermented food. Food Research International 37, 123-131   DOI   ScienceOn
11 Zhang, K., Wu, S., Tang, X., Kaiser, N. K., and Bruce, J. E. (2007), A bifunctional monolithic column for combined protein preconcentration and digestion for high throughput proteomics research. Journal of Chromatography B 849, 223-230   DOI   ScienceOn
12 Kim, B. C., Nair, S., Kim, J., Kwak, J. H., Grate, J. W., Kim, S. H., and Gu, M. B. (2005), Preparation of biocatalytic nanofibres with high activity and stability via enzyme aggregate coating on polymer nanofibres. Nanotechnology 16, S382-S388   DOI   ScienceOn
13 Gupta, A. K. and Gupta, M. (2005), Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications. Biomaterials 26, 3995-4021   DOI   ScienceOn
14 Kim, J. and Grate, J. W. (2003), Single-enzyme nanoparticles armored by a nanometer-scale organic/inorganic network. Nano Letters 3, 1219-1222   DOI   ScienceOn