Browse > Article

In the presence of organic solvent stability of CiP [coprinus cinereus peroxidase]  

Kim, Han-Sang (Department of Chemical Engineering, Kwangwoon University)
Cho, Dae-Haeng (Department of Chemical Engineering, Kwangwoon University)
Kim, Yong-Hwan (Department of Chemical Engineering, Kwangwoon University)
Publication Information
KSBB Journal / v.23, no.4, 2008 , pp. 340-344 More about this Journal
Abstract
Coprinus cinereus peroxidase (CiP) was often used as a catalyst for oxidative polymerization of a variety of phenol derivatives to produce a new class of polyphenols. Economical point of view, to know the mechanism of enzyme deactivation is significantly important because cost of enzyme is critically high. Hydrogen peroxide being used as oxidizing agent induced deactivation of peroxidase by destruction of heme structure. In the presence of hydrogen peroxide the stability of peroxidase was unexpectedly improved by adding organic solvent. Especially 2-propanol significantly improved enzyme stability among tested solvents. Radical scavenging by organic solvents may play a major role in protecting peroxidase from the oxidation of oxidizing radicals.
Keywords
Coprinus cinereus peroxidase; peroxidsae; enzyme stability; organic solvent; hydrogen peroxide;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Kim, Y. H. and Song, B. K. (2007), Polymerization of bisphenol a using Coprinus cinereus peroxidase and ist application as a photoresist resin, J. Mol. Catalysis B 44, 149-154   DOI   ScienceOn
2 A. Cui, A. Singh, and D. L. Kaplan, (2002), Enzyme-Based Molecular Imprinting with Metals, Biomacromolecules. 3, 1353-1358   DOI   ScienceOn
3 Valderrama, B. and Ayala, M. and Vazquez-Duhalt, R. (2002). Suicide inactivation of peroxidase and the challenge of engineering more robust enzymes, Chem Biol. 9, 555-565   DOI   ScienceOn
4 J. A. Akkara, K. J. Senecal, and D. L. Kaplan (1991). Synthesis and characterization of polymers produced by horseradish peroxidase in dioxane, J. Polym Sic A Polym Chem. 29, 1561   DOI
5 Y. Pocker and N. Janjic, (1987), Enzyme kinetics in solvent of increased viscosity. Dynamic aspects of carbonic anhydrase catalysis, Biochemistry 26, 2597-2606   DOI   ScienceOn
6 A. Brant and J. Richard (2004), Idenfication of the reactive oxygen species responsible for carbon tetrachloride degradation in moified fenton's system, Envrion sci techol. 38, 5465-5469   DOI   ScienceOn
7 Z. Xia, T. Yoshida, and M. Funaoka. (2003), Enzymatic synthesis of polyphenols from highly phenolic lignin-based polym ers (lignophenols), Biotechnol. Lett. 25, 9-12   DOI   ScienceOn
8 C. M. Miler, K. C. Bower, and C. Rise, (2003), Fenton's reagent degration of 2,4-dinitrotoluene in water-acetone mixture, Environ eng sci. 2, 65-69
9 Y. H. Kim, Won, J. M. Kwon, H. S. Jeong, S. Y. Park, E. S. An, and B. K. Song, (2005), Synthesis of polycardanol from renewable source using a fungal peroxidase Coprinus cinereus, J. Molecular Catalyst B. Enzymatic 34, 33-38   DOI   ScienceOn
10 J. S. Dordick, H. Marletta, and A. M. Klivanov (1986), Peroxidases depolymerize lignin in organic media but not in water 83, 6255-6257
11 H. Tonami, H. Uyama, S. Kobayashi, K. Rettig, and H. Ritter, (1999). Chemoenzymatic synthesis of a poly (hydroquinone), Marcromol Chem Phys, 200, 1998-2002   DOI
12 J. Kadota, T. Fukuoka, H. Uyama, K. Hasegawa, and S. Kobayashi, (2002), New Positive-Type Photoresists Based on Enzymatically Synthesized Polyphenols, Macromol. Rapid Commun. 25, 441-444   DOI   ScienceOn
13 M. Akita, D. Tsutumi, M. Kobayashi, and H. Kise, (2001), Structural change and catalytic activity of horseradish peroxidase in oxidative polymerization of phenol, Biotechol. Biochem 65, 1581-1588   DOI   ScienceOn
14 J. Marugan and D. Hufschmidt, (2006), Photonic efficiency for methanol photooxidation and hydroxyl radical generation on silica-supported TiO2 photocatalysts, Applied catalysis B 62, 201-207   DOI   ScienceOn
15 Grey, C. E. and Adlercreutz, P. (2007), A mass spectrometic investigation of native and oxidatively inactivated chloroperoxidase, ChemBio Chem. 8, 1055-1062   DOI   ScienceOn
16 H. Tonami, H. Uyama, S. Kobayashi, and M. Kubota, (199), Peroxidase-catalyzed oxidative polymerization of m-substitued phenol derivates, Marcromol Chem Phys. 200, 2365-2371   DOI
17 Park, J. B. and Clark D. S. (2006), Deactivation mechanism of chloroperoxidase during biotransformations, Biothenology and Bioengineering, 93(6)
18 K. Ryu and J. S. Dordick (1992), How do organic solvents affects peroxidase structure and function?, Biochemistry 31, 2588-2598   DOI