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Urease Inhibition and Flocculating Activity of Concentrated Aloe vera Gel by Using Ultrafiltration Process  

Baek, Jin-Hong (KJM Aloe R&D Center)
Kim, Sung-A (KJM Aloe R&D Center)
Lee, Shin-Young (Department of Bioengineering and Technology, Kangwon National University)
Publication Information
KSBB Journal / v.23, no.3, 2008 , pp. 239-244 More about this Journal
Abstract
For physiological function of aloe concentrate by ultrafiltration (UF) process, jack bean urease inhibitory activity and bentonite flocculating activity of UF aloe concentrate was investigated and compared with fresh aloe gel. Urease inhibitory activity of UF aloe concentrate ranged from 87 to 90% in 1 mL sample. Also, urease inhibitory activity of UF aloe concentrate increased about 10% by heat treatment showing the heat stability. From Lineweaver-Burk plot for UF aloe concentrate, urease inhibition pattern indicated general non-competitive inhibition. From flocculation test of UF aloe concentrate about 1% (w/v) bentonite suspension, maximum flocclulating activity of 97% was obtained at 0.5 mL addition of UF aloe concentrate/ 5 ml bentonite suspension. However, flocculating activity of 81% was obtained at 1 mL addition of UF aloe concentrate/ 5 mL bentonite suspension, which was typical flocculating behavior of polymers with re-dispersion at overdose area. FT-IR spectra of UF aloe concentrate showed the characteristic patterns of $\beta$-binding polysaccharide and less deacetylation indicating higher level of bioactive polysaccharide content.
Keywords
Aloe vera gel; ultrafiltration; concentration; polysaccharide; urease inhibition; flocculating activity;
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