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Isolation and Characterization of Microorganisms with Broad Antifungal Activity against Phytopathogenic Fungi  

Kim, Min-Hee (Department of Microbiology, College of Natural Science, Keimyung University)
Ko, Hee-Sun (Department of Microbiology, College of Natural Science, Keimyung University)
Yook, Young-Min (Keimyung Biotech Co.)
Kim, Hyun-Soo (Department of Microbiology, College of Natural Science, Keimyung University)
Publication Information
KSBB Journal / v.23, no.3, 2008 , pp. 219-225 More about this Journal
Abstract
For the production of an antifungal compound, one strain (I-8) was selected from approximately 400 strains isolated from various soil samples. The optimum carbon source, nitrogen source and pH culture conditions for the production of the antifungal compound were investigated. ISP No. 2 medium (yeast extract 0.4%, malt extract 1% and dextrose 0.4%, at pH 8) was determined to be the optimum medium. Strain I-8 showed broad antifungal activity against the plant pathogenic fungi tested, including Sclerotinia sclerotiorum KACC 41065, as well as cellulase and chitinase activities in an agar plate assay. The extraction of antifungal compounds was performed using ethyl ether and ethyl acetate. In a culture broth of strain I-8, the ethyl acetate extract exhibited effective growth inhibition against 14 of the 20 phytopathogenic fungi tested. By mixing the ethyl acetate extract from I-8 with the ethyl ether extract from the fungus 13-16, which shows specific antifungal activity against Colletotrichum orbiculare KACC 40808, the antifungal activity of I-8 against phytopathogenic fungi was confirmed to be slightly increased. Strain I-8 showed strong growth inhibition against 16 phytopathogenic strains in agar plate tests.
Keywords
Antifungal compound; chitinase; cellulase; phytopathogenic fungi;
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Times Cited By KSCI : 9  (Citation Analysis)
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