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Isolation of lysozyme producing bacteria capable of solubilizing microbial cells  

Guo, Pengfei (Department of Environmental Engineering and Biotechnology, Myongji University)
Seo, Sun-Keun (Department of Environmental Engineering and Biotechnology, Myongji University)
Zhang, Lei (Department of Environmental Engineering and Biotechnology, Myongji University)
Kim, Hyo-Sang (GS E&C Research Institute)
Oh, Young-Khee (GS E&C Research Institute)
Jahng, Deok-Jin (Department of Environmental Engineering and Biotechnology, Myongji University)
Publication Information
KSBB Journal / v.23, no.3, 2008 , pp. 187-192 More about this Journal
Abstract
Lysozyme-producing microorganisms were isolated to obtain bacteria which can efficiently solubilize microbial cells. Cells of normal and chloroform-treated Escherichia coli and Micrococcus Iysodeikticus were used as model substrates to isolate lysozyme-producing microorganisms and investigate the efficiency of cell lysis. The culture supernatant of the isolate New1 (98% similarity of 16S rDNA sequence with Thermomonas haemolytica) showed different lytic characteristics for different substrates. Thermal treatment (autoclave) of substrate cells showed a significant effect on cell solubilization by culture supernatant of the New1. For autoclaved substrate cells, E. coli, M. Iysodeikticus and chloroform-treated E. coli were solubilized by 58.7%, 49.4% and 79.1%, respectively, in the culture supernatant of New1. The lytic activity of New1 was mainly caused by lysozyme produced by the isolate. It was also showed that New1 exhibited high protease activity and a little cellulase activity.
Keywords
chloroform-treated E. coli cells; lysozyme-producing bacteria; lytic activity;
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