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Biological activities of ethanol extract from the seawater algae, Chlorella elliposidea C020  

Kim, Hyun-Jin (Department of Pharmaceutical Engineering, Collage of Medial Life Science, Silla University)
Kim, In-Hae (Department of Pharmaceutical Engineering, Collage of Medial Life Science, Silla University)
Lee, Jae-Hwa (Department of Pharmaceutical Engineering, Collage of Medial Life Science, Silla University)
Publication Information
KSBB Journal / v.23, no.2, 2008 , pp. 125-130 More about this Journal
Abstract
We investigated the biological activities of ethanol extract from the seawater algae, Chlorella elliposidea C020 such as antibacterial activity, anti-oxidant activity, tyrosinase inhibitory activity and hemolytic activity against human erythrocytes. Extract was obtained from various solvent, methanol, ethanol, acetone and ethanol + acetone (1:1, v/v%), 95% ethanol proved to be best extraction solvents. The contents of ethanol extract were higher in freeze-dried sample than that in frozen-thawing. Antibacterial activities of ethanol extract showed strong inhibitory effect against Bacillus subtilis PM125, Bacillus licheniformis and fish pathogenic bacteria, Vibrio parahaemolyticus KCTC2471 and Edward tarda NUF251. However, this extract didn't worked against antifungal activity against Candida albicans KCTC1940. And, ethanol extract was without hemolytic activity against human erythorocytes. The ethanol extract showed 75% of free radical scavanging effect on 2.0 mg/mL using DPPH method. In tyrosinase inhibition assay of ethanol extract, $IC_{50}$ (Inhibition Concentration) was measured as 10.87 mg/mL. Conclusionally, ethanol extract of Chlorella elliposidea C020 has good candidate for bioactive materials.
Keywords
Chlorella elliposidea C020; antibacterial activity; antioxidant activity; DPPH; tyrosinase inhibitory activity;
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