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Development of a Biosensor Using Electrochemically-Active Bacteria [EAB] for Measurements of BOD [Biochemical Oxygen Demand]  

Yoon, Seok-Min (Department of Microbial Engineering, Konkuk University)
Choi, Chang-Ho (Department of Microbial Engineering, Konkuk University)
Kwon, Kil-Koang (Department of Microbial Engineering, Konkuk University)
Jeong, Bong-Geun (Department of Microbial Engineering, Konkuk University)
Hong, Seok-Won (Center for Environmental Technology Research, Korea Institute of Science and Technology)
Choi, Yong-Su (Center for Environmental Technology Research, Korea Institute of Science and Technology)
Kim, Hyung-Joo (Department of Microbial Engineering, Konkuk University)
Publication Information
KSBB Journal / v.22, no.6, 2007 , pp. 438-442 More about this Journal
Abstract
A biosensor using electrochemically-active bacteria (EAB) enriched in three-electrode electrochemical cell, was developed for the determination of biochemical oxygen demand (BOD) in wastewater. In the electrochemical cell, the positively poised working electrode with applying a potential of 0.7 V was used as an electron acceptor for the EAB. The experimental results using artificial and raw wastewater showed that the current pattern generated by the biosensor and its Coulombic yield were proportional to the concentration of organic matter in wastewater. The correlation coefficients of BOD vs Coulombic yield and $BOD_5$ vs Coulombic yield were 0.99 and 0.98, respectively. These results indicate that the biosensor enriched with the EAB capable of transferring electrons directly toward the electrode can be utilized as a water-quality monitoring system due to a quick and accurate response.
Keywords
BOD biosensor; three-electrode system; electrochemically-active- bacteria (EAB); electrochemical enrichment;
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Times Cited By KSCI : 3  (Citation Analysis)
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