Browse > Article

Development of an Open Sandwich Fluoroimmunoassay Based on FRET  

Wei, Quande (Department of Pathogenic Biology, Sun Yat-sen University)
Lee, Moon-Kwon (Micro Biochip Center, Hanyang University)
Seong, Gi-Hun (Micro Biochip Center, Hanyang University)
Choo, Jae-Bum (Micro Biochip Center, Hanyang University)
Lee, Eun-Kyu (Micro Biochip Center, Hanyang University)
Publication Information
KSBB Journal / v.22, no.6, 2007 , pp. 426-432 More about this Journal
Abstract
We have developed a sensitive, one-step, homogeneous open sandwich fluoroimmunoassay (OsFIA) based on fluorescence resonance energy transfer (FRET) and luminescent semiconductor quantum dots (QDs). In this FRET assay, estrogen receptor-$\beta$ (ER-$\beta$) antigen was incubated with QD-labeled anti-ER-$\beta$ monoclonal antibody and AF (Alexa Fluoro)-labeled anti-ER polyclonal antibody for 30 minutes, followed by FRET measurement. The dye separation distance was estimated to be between $80\sim90\;{\AA}$. The present method is rapid, simple and highly sensitive, and did not require the bound/free reagent separation steps and solid-phase carriers. A concentration as low as 0.05 nM (2.65 ng/ml) receptor was detected with linearity ($R^2$ > 0.990). In addition, the assay was performed with commercial antibodies. This assay provides a convenient alternative to conventional, laborious sandwich immunoassays.
Keywords
FRET; sandwich assay; quantum dots; fluorescence labels; sandwich immunoassay;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Ruiz-Velasco, V. and S. R. Ikeda (2001), Functional expression and FRET analysis of green fluorescent proteins fused to G-protein subunits in rat sympathetic neurons, J. Physiol. 537, 679-692   DOI
2 Bruchez, J. M., M. Moronne, P. Gin, S. Weiss, and A. P. Alivisatos (1998), Semiconductor nanocrystals as fluorescent biological labels, Science 281, 2013-2016   DOI
3 Wang, S., N. Mamedova, N. A. Kotov, W. Chen, and J. Studer (2002), Antigen/antibody immunocomplex from CdTe nanopartic1e bioconjugates, Nano Letters 2, 817-822   DOI   ScienceOn
4 Medintz, I. L., J. H. Konnert, A. R. Clapp, I. Stanish, M. E. Twigg, H. Mattoussi, and J. M. Mauro (2004), A fluorescence resonance energy transfer-derived structure of a quantum dot-protein bioconjugate nanoassembly, Proc. Natl. Acad. Sci. USA 101, 9612-9617
5 Wang, G., J. Yuan, K. Matsumoto, and Z. Hu (2001), Homogeneous time-resolved fluoroimmunoassay of bensulfuron-methyl by using terbium fluorescence energy transfer, Talanta 55, 1119-1125   DOI   ScienceOn
6 Youn, H. J., E. Terpetschnig, H. Szmacinski, and J. R. Lakowicz (1995), Fluorescence energy transfer immunoassay based on a long-lifetime luminescent metal -ligand complex, Anal. Biochem. 232, 24-30   DOI   ScienceOn
7 Selvin, P. R. (2000), The renaissance of fluorescence resonance energy transfer, Nat. struct. Biol. 7, 730-734   DOI   ScienceOn
8 Pulli, T., M. Hoyhtya, H. Soderlund, and K. Takkinen (2005), One-step homogeneous immunoassay for small analytes, Anal. Chem. 77, 2637-2642   DOI   ScienceOn
9 Mattoussi, H., J. M. Mauro, E. R. Goldman, G. P. Anderson, V. C. Sundar, F. V. Mikulec, and M. G. Bawendi (2000), Self-assembly of CdSe-ZnS quantum dot bioconjugates using an engineered recombinant protein, J. Am. Chem. Soc. 122, 12142-12150   DOI   ScienceOn
10 Fehr, M., W. B. Frommer, and S. Lalonde (2002), From the Cover: Visualization of maltose uptake in living yeast cells by fluorescent nanosensors, Proc. Natl. Acad. Sci. USA 99, 9846-9851
11 Lundin, K., K. Blomberg, T. Nordstrom, and C. Lindqvist (2001), Development of a time-resolved fluorescence resonance energy transfer assay (Cell TR-FRET) for protein detection on intact cells, Anal. Biochem. 299, 92-97   DOI   ScienceOn
12 Mattoussi, H., I. L. Medintz, A. R. Clapp, E. R. Goldman, J. K. Jaiswal, S. M. Simon, and J. M. Mauro (2004), Luminescent quantum dot-bioconjugates in immunoassays, FRET, biosensing and imaging applications, JALA 9, 28-32   DOI   ScienceOn
13 Suzuki, C., H. Ueda, K. Tsumoto, W. Mahoney, I. Kumagai, and T. Nagamune (1999), Open sandwich ELISA with$V_H$-/$V_L$-alkaline phosphatase fusion proteins, J. Immunol. Methods 224, 171-184   DOI   ScienceOn
14 Sapsford, K. E., I. L. Medintz, J. P. Golden, J. R. Deschamps, H. T. Uyeda, and H. Mattoussi (2004), Surface-immobilized self-assembled protein-based quantum dot nanoassemblies, Langmuir 20, 7720-7728   DOI   ScienceOn
15 Selvin, P. R. (1995), Fluorescence resonance energy transfer, Methods Enzymol. 246, 300-334   DOI
16 Tran, P. T., E. R. Goldman, G. P. Anderson, J. M. Mauro, and H. Mattoussi (2002), Use of luminescent CdSe-ZnS nanocrystal bioconjugates in quantum dot-based nanosensors, Phys. Stat. Sol. B. 229, 427-432.   DOI   ScienceOn
17 Goldman, E. R., A. R. Clapp, G. P. Anderson, H. T. Uyeda, J. M. Mauro, I. L. Medintz, and H. Mattoussi (2004), Multiplexed toxin analysis using four colors of quantum dot fluororeagents, Anal Chem. 76, 684-688   DOI   ScienceOn
18 Arai, R., H. Nakagawa, K. Tsumoto, W. Mahoney, I. Kumagai, H. Veda, and T. Nagamune (2001), Demonstration of a homogeneous noncompetitive immunoassay based on bioluminescence resonance energy transfer, Anal. Biochem. 289, 77-81   DOI   ScienceOn
19 Alivisatos, A. P. (1996), Semiconductor clusters, nanocrystals, and quantum dots, Science 271, 933-937   DOI   ScienceOn
20 Clegg, R. M. (2002), FRET tells us about proximities, distances, orientations and dynamic properties, J. Biotechnol. 82, 177-179
21 Chan, W. and S. Nie (1998), Quantum dot bioconjugates for ultrasensitive nonisotopic detection, Science 281, 2016-2018   DOI
22 Clegg, R. M. (1995), Fluorescence resonance energy transfer, Curr. Opin. Biotechnol. 6, 103-110   DOI   ScienceOn
23 Van Thor, J. J. and K. J. Hellingwerf (2002), Fluorescence resonance energy transfer (FRET) applications using green fluorescent protein. Energy transfer to the endogenous chromophores of phycobilisome light-harvesting complexes, Methods Mol. Biol. 183, 101-119
24 Veda, H., K. Kubota, Y. Wang, K. Tsumoto, W. Mahoney, I. Kumagai, and T. Nagamune (1999), Homogeneous noncompetitive immunoassay based on the energy transfer between fluorolabeled antibody variable domains (Open Sandwich Fluoroimmunoassay), Biotechniques 27, 738-742
25 Oswald, B., F. Lehmann, L. Simon, E. Terpetschnig, and O. S. Wolfbeis (2000), Red laser-induced fluorescence energy transfer in an immunosystem, Anal. Biochem. 280, 272-277   DOI   ScienceOn
26 Clapp, A. R., I. L. Medintz, J. M. Mauro, B. Fisher, M. G. Bawendi, and H. Mattoussi (2004), Fluorescence resonance energy transfer between quantum dot donors and dye-labeled protein acceptors, J. Am. Chem. Soc. 126, 301-310   DOI   ScienceOn
27 Hillisch, A., M. Lorenz, and S. Diekmann (2001), Recent advances in FRET: distance determination in protein - DNA complexes, Curr. Opin. Struct. Biol. 11, 201-207   DOI   ScienceOn
28 Periasamy, A. and R. N. Day (1999), Visualizing Protein Interactions in living cells using digitized GFP imaging and FRET microscopy, Methods Cell Biol. 58, 293-311   DOI