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Anti-angiogenic and Anti-cell Adhesion Effects and Their Mechanism with the Extract of Camellia japonica Leaf  

Song, Min-Gyu (Lab. of Biochemistry, College of Medicine, DongA University)
Seo, Hyo-Jin (Green Biotech Co., Ltd.)
Moon, Je-Hak (Department of Food Science and Technology and Funtional Food Research Center, Chonnam National University)
Park, Keun-Hyung (Department of Food Science and Technology and Funtional Food Research Center, Chonnam National University)
Kim, Jong-Deog (Department of Biotechnology Chonnam National University)
Publication Information
KSBB Journal / v.22, no.4, 2007 , pp. 249-254 More about this Journal
Abstract
Anti-angiogenesis and anti-cell adhesion effects were investigated with different dose of Camellia japonica leaf (CJL) extract for applying anti-cancer, anti-metastasis and anti-obesity. Cytotoxicity on HUVECs was very low at 200 ug/mL of CJL-extract. Anti-angiogenic ratio at increasing dose of 1.5 ug/mL, 3.0 ug/mL, 15 ug/mL and 30 ug/mL was showed 30.7%, 38.5%, 53.8%, and 70.0%, respectively. Also, anti-cell adhesion effect at concentration of $50{\mu}g{/well},\;100{\mu}g{/well}\;and\;200{\mu}g{/well}$ was expressed on E-selectin by 46.7%, 66.7%, and 86.76%, on VCAM-1, 23.0%, 61.5%, and 84.6%, and on ICAM-1, 11%, 55.5%, and 88.8%, respectively. For inquiring anti-angiogenesis mechanism, when western blot was performed with different dose of CJL extract, signal molecules of VEGFR-2, $\beta$-catenin and PI3-K were suppressed. As the signal transduction from VEGFR-2, $\beta$-catenin and PI3-K to NF-${\kappa}$B was interupted, angiogenesis could not be occurred causing not activated NF-kB. C. japonica leaf (CJL) is a useful herb for developing therapeutics of angiogenesis related diseases such as cancer, metastasis, rheumathioid arthritis and obesity.
Keywords
Camellia japonica leaf (CJL); angiogenesis; cell adhesion; signal molecules; anti-obesity;
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