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Octimization of Conditions of Filtration and Concentration of Methanol Extract for Recovery of Paclitaxel from Plant Cell Culture  

Kim, Jin-Hyun (Department of Chemical Engineering, Kongju National University)
Publication Information
KSBB Journal / v.22, no.4, 2007 , pp. 197-200 More about this Journal
Abstract
This study examined the conditions of filtration and concentration of methanol extract from biomass. Filtration efficiency was improved by adding diatomaceous earth as a filter aid. The optimal amount of diatomaceous earth was 6% (w/w) to reduce the filtration time. The filtration time was reduced by 4.2% in first extraction, 30.0% in second extraction, 22.8% in third extraction, and 19.0% in fourth extraction, respectively. The optimal temperature of water bath was below 50$^{\circ}C$ for preventing paclitaxel degradation during concentration of methanol extract using a rotary evaporator. The temperature of concentrated solution in rotary evaporator was relatively low compared to bath temperature because of latent heat of evaporation. The stopping point of concentration in rotary evaporator for the following step was at a specific gravity of 0.96 of the concentrated solution in terms of the purity and yield of paclitaxel. This information is very useful for mass extraction of biomass for the recovery of paclitaxel from plant cell culture.
Keywords
Paclitaxel; extraction; filtration; diatomaceous earth; concentration;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 Choi, H. K., S. J. Son, G. H. Na, S. S. Hong, Y. S. Park, and J. Y. Song (2002), Mass production of paclitaxel by plant cell culture, Korean J. Plant Biotechnol. 29, 59-62   DOI
2 Pyo, S. H., H. B. Park, B. K. Song, B. H. Han, and J. H. Kim (2004) A large-scale purification of paclitaxel from cell cultures of Taxus chinensis, Process Biochem. 39, 1985-1991   DOI   ScienceOn
3 Kim, J. H. and S. S. Hong (2000), Optimization of extraction process for mass production of paclitaxel from plant cell cultures, Kor. J. Biotechnol. Bioeng. 15, 346-351   과학기술학회마을
4 Belter, P. A., E. L. Cussler, and W. -S. Hu (1988), Bloseparations, 1st ed., p13, John Wiley & Sons, New York
5 Kim, J. H. (2004), Prepurification of paclitaxel by micelle and precipitation, Process Biochem. 39, 1567-1571   DOI   ScienceOn
6 Witherup, K. M., S. A. Look, M. W. Stasko, T. J. Ghiorzi, G. M. Muschik, and G. M. Cragg (1990), Texus spp. needles contain amounts of taxol comparable to the bark of Taxus brevifolia: analysis and isolation, J. Nat. Prod. 53, 1249-1255   DOI
7 Rao, K. V., J. R. Hanuman, C. Alvarez, M. Stoy, J. Juchum, R. M. Davies, and R. Baxley (1995), A new large-scale process for taxol and related texancs from Taxus brevifolia, Pharm. Res. 12, 1003-1010   DOI
8 Pyo, S. H., D. K Song, C. H. Ju, D. H. Han, and H. J. Choi (2005), Effects of adsorbent treatment on the purification of paclhaxel [rom cell cultures of Taxus chinensis and yew tree, Process Biochem. 40, 1113-1117   DOI   ScienceOn
9 Castor, T. P. and T. A. Tyler (1993), Determination of taxol in Taxus media needles in the presence of Interfering compounds, J. Liq. Chromatogr. 16, 723-731   DOI   ScienceOn
10 Choi, H. K., T. L. Adams, R. W. Stahlhut, S. I. Kim, J. H. Yun, D. K. Song, J. H. Kim, S. S. Hong, and H. S. Lee (1999) Method for mass production of taxol by semi-continuous culture with Taxus chinensis cell culture, U.S.Patent 5,871,979
11 Kim J. H., I. S. Kang, H. K. Chol, S. S Hong, and H. S. Lee (2002), A novel prepurification for paclitaxel from plant cell cultures, Process Biochem, 37, 679-682   DOI   ScienceOn
12 Kim, J. H. (2006), Paclitaxel recovery and purification in commercialization step, Kor. J. Biotechnol. Bioeng. 21, 1-10   과학기술학회마을
13 Baloglu, F. and D. G. Kingston (1999), A new semisynthesis of paclitaxel from baccatin III, J. Nat. Prod. 62, 1068-1071   DOI   ScienceOn