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Isolation and Characterization of An Alcohol Fermentation Strain from Anaerobic Acid Fermentor to Treat Food Wastes  

Kim, Jung-Kon (Department of Bio Materials Engineering, Chosun University)
Han, Gui-Hwan (Department of Environmental Engineering, Chosun University)
Yoo, Jin-Cheol (Department of Pharmacy, Chosun University)
Seong, Chi-Nam (Department of Biology, Sunchon National University)
Kim, Seong-Jun (Department of Civil, Geosystem and Environmental Engineering, Chonnam National University)
Kim, Si-Wouk (Department of Environmental Engineering, Chosun University)
Publication Information
KSBB Journal / v.21, no.6, 2006 , pp. 451-455 More about this Journal
Abstract
An efficient pilot scale (10 ton) three-stage methane fermentation system to digest food waste has been developed in this laboratory. This system consisted of three stages: semianaerobic hydrolysis, anaerobic acidogenesis and strictly anaerobic methanogenesis. From the secondary acidogenesis reactor, a novel strain KA4 responsible for alcohol fermentation was isolated and characterized. The cell was oval and its dimension was $5.5-6.5{\times}3.5-4.5\;{\mu}m$. This strain was identified as Saccharomyces cerevisiae KA4 by 26S rDNA D1/D2 rDNA sequence. Optimal culture temperature was $30-35^{\circ}C$. Cells were tolerant to 5% (v/v) ethanol concentration, however, were inhibited significantly by higher ethanol concentration up to 7%. The strain could grow well up to 50% (w/v) initial glucose concentration in the YM liquid medium, however, optimal concentration for ethanol fermentation was 10%. It could produce ethanol in a broad initial pH range from 4 to 10, and optimal pH was 6. In this condition, the strain converted 10% glucose to 7.4% ethanol during 24 hr, and ethanol yield was estimated to be 2.87 moi EtOH/mol glucose.
Keywords
Alcohol fermentation; Saccharomyces cerevisiae KA4; three-stage methane fermentation system;
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1 Kim, S. and B. E. Dale (2004), Global potential bioethanol production from wasted crops and crop residues, Biomass Bioenerg. 26(4), 361-375   DOI   ScienceOn
2 Sun, Y. and J. Cheng (2002), Hydrolysis of lignocellulosic materials for ethanol ptoduction: a review, Bioresource Technol. 83, 1-11   DOI   ScienceOn
3 Kim, S. W., J. Y. Park, J. K. Kim, J. H. Cho, Y. N. Chun, I. H. Lee, J. S. Lee, J. S. Park, and D. H. Park (2000), Development of a modified three-stage methane ptoduction process using food wastes, Appl. Biochem. Biotech. 84-86,731-741
4 Kim, J. K., J. H. Cho, J. S. Lee, K. S. Hahm, D. H. Park, and S. W. Kim (2002), Mass production of methane from food wastes with concomitant wastewater treatment. Appl. Biochem. Biotech. 98-100,753-764
5 Ohta, K., K. Supanwong, and S. Hayashida (1981), Environmental effects on ethanol tolerance of Zymomonas mobilis, Annual Reports ICME. 3, 109-116
6 Kadar, Z., Z. Szengyel, and K. Reczey (2004), Simultaneous saccharification and fermentation (SSF) of industrial wastes for the production of ethanol, Ind. Crop Prod. 20, 103-110   DOI   ScienceOn
7 Shapouri, H., J. A. Duffield, and M. Wang (2002), The energy balance of com ethanol: an update. Agricultural Economic Report 813, United States Department of Agricultute
8 Zafar, S. and M. Owais (2006), Ethanol ptoduction from crude whey by Kluyveromyces marxianus, Biochem. Eng. J. 27, 295-298   DOI   ScienceOn
9 Shapouri, H., J. A. Duffield, and M. S. Graboski (1995), Estimating the net energy balance of com ethanol, Agricultutal Economic Report 721, United States Department of Agricultute
10 Miller, G. L. (1959), Use of dinitrosalicylic acid reagent for determination of reducing sugar, Anal. Chem. 31, 426-428   DOI
11 Jones, M. A. and W. M. mgledew (1994), Fuel alcohol production: optimization of temperatute for efficient very-high-gtavity fermentation, Appl. Environ. Microbiol. 60, 1048-1051
12 Borrero, M. A. V., J. T. V. Pereira, and E. E. Miranda (2003), An environmental management method for sugar cane alcohol production in Brazil, Biomass Bioenerg. 25(3), 287-299   DOI   ScienceOn
13 Murphy, J. D. and K. McCarthy (2005), Ethanol production from energy crops and wastes for use as a transport fuel in Ireland, Appl. Energ. 82, 148-166   DOI   ScienceOn
14 Hur, B. K., J. S. Yu, and J. W. Yang (1989), Ethanol fermentation by Kluyveromyces fragilis from Jerusalem artichoke, Kor. J. Biotechnol. Bioeng. 4, 50-58
15 Bajpai, P. K. and A. Margaritis (1986), Effect of temperatute and pH on immobilized Zymomonas mobilis for continuous ptoduction of ethanol, Biotech. Bioeng. 28, 824-828   DOI   ScienceOn
16 Lynd, L. R, J. H. Cushman, R. J. Nichols, and C. E. Wyman (1991), Fuel ethanol from cellulosic biomass, Science 251, 1318-1323   DOI   ScienceOn