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The DeveloDment of PaDain which is Extremely Stable to Negative Ionic Environment by Directed Molecular Evolution  

Kang, Whan-Koo (Department of Chemical Engineering & Nano-Bio Technology, The University of Hannam)
Hwang, Sun-Duk (Daecheong bldg.)
Kim, Hyoung-Sik (Daecheong bldg.)
Jeung, Jong-Sik (Department of Chemical Engineering & Nano-Bio Technology, The University of Hannam)
Lee, Bheong-Uk (Department of Biological Sciences, The University of Kosin)
Publication Information
KSBB Journal / v.21, no.5, 2006 , pp. 394-400 More about this Journal
Abstract
When the papain, which is a sort of Cystein protease, is applied to the outer skin, it decomposes the protein which forms the peeled outer skin and speeds up metabolism. Therefore, it is one of the most important cosmetics compositic which keeps the function of skin normal. When the papain is used in cosmetics with surfactant, the activity of papain is reduced rapidly. In this study, the modified papain with extreme stability negative ionic environment was developed by directed evolution
Keywords
Cystein protease; papain; error prone PCR; mutagenesis; staggered extension process; toiletry dopes enzyme;
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