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Rapid Preparation of Truncated Transaminases using a PCR-based Cell-free Protein Synthesis System  

Kwon, Yong-Chan (Department of Fine Chemical Engineering and Chemistry, School of Engineering, Chungnam National University)
Park, Kyung-Moon (Department of Chemical System Engineering, Hongik University)
Kim, Dong-Myung (Department of Fine Chemical Engineering and Chemistry, School of Engineering, Chungnam National University)
Publication Information
KSBB Journal / v.21, no.4, 2006 , pp. 302-305 More about this Journal
Abstract
In this work, we attempted the application of cell-free protein synthesis technology for the rapid generation of truncated enzymes. Truncated DNAs of a transaminase were PCR-amplified and directly expressed in cell-free protein synthesis reactions. Variants of the transaminase were rapidly prepared and analyzed for their enzymatic activity. Described method that combines the PCR and cell-free protein synthesis technologies will offer a versatile platform for the rapid generation of optimally modified protein species.
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