Browse > Article

Production of Recombinant Trehalose Synthase from Thermus caldophilus GK24  

Choi, Jae-Youl (EnzBank, Inc., Bioventure Center(BVC), Korea Research Institute of Bioscience and Biotechnology(KRIBB))
Cha, Wol-Suk (Department of Chemical and Biochemical Engineering, Chosun University)
Shin, Hyun-Jae (Department of Chemical and Biochemical Engineering, Chosun University)
Publication Information
KSBB Journal / v.21, no.4, 2006 , pp. 298-301 More about this Journal
Abstract
A gene(GeneBank AF 135796) coding for a trehalose synthase from Thermus caldophilus GK24 was cloned into Escherichia coli K12 using five vector systems. The constitutive expression system(pHCETS) which shows the highest trehalose synthase activity from flask culture of recombinant E. coli was selected for the production of trehalose from maltose. For the shake flask culture, the final dry cell weight was 0.9 g/L and the trehalose synthase activity was 25 U/mL. Fed-batch culture of recombinant E. coli harboring plasmid pHCETS which uses the glycerolas a carbon source was performed in jar fermentor: the dry cell weight of 20 g/L and the trehalose synthase activity of 13.7 U/mL were attained in 48 h.
Keywords
Trehalose; trehalose synthase; Thermus caldophilus GK24; constitutive expression vector; fed-batch culture;
Citations & Related Records
연도 인용수 순위
  • Reference
1 De Virgilio, C. (1994), The role of trehalose synthesis for the acquisition of thermotolerance in yeast, Eur. J. Biochem. 219, 179-186   DOI   ScienceOn
2 Waugh, D. S. (2005), Making the most of affinity tags, TIBTECH. 23, 316-320   DOI   ScienceOn
3 Koh, S., J. S. Kim, H. -J. Shin, D. H. Lee, J. D. Bae, D. I. Kim, and D. S. Lee (2003), Mechanistic study of the intramolecular conversion of maltose to trehalose by Thermus caldophilus GK24 trehalose synthase, Carbohydr. Res. 338, 1339-1343   DOI   ScienceOn
4 Zhang, X., P. Guo, and G. Jing (2003), A vector with the downstream box of the initiation codon can highly enhance protein expression in Escherichia coli, Biotechnol. Lett. 25, 755-760   DOI   ScienceOn
5 Trevelyan, W. E. (1956), Studies on yeast metabolism. The trehalose content of baker's yeast during anaerobic fermentation, Biochem. J. 62, 177-182   DOI
6 Koh, S., H. -J. Shin, D. S. Lee, and S. Y. Lee (1998), Trehalose synthesis from maltose by a thermostable trehalose synthase from Thermus caldophilus, Biotechnol. Lett. 20, 757-761   DOI   ScienceOn
7 Nishimoto, T., M. Nakano, T. Nakada, H. Chaen , S. Fukuda, T. Sugimoto, M. Kurimoto, and Y. Tsujisaka (1996), Purification and properties of a novel enzyme, trehalose synthase, from Pimelobacter sp. R48, Biosci. Biotech. Biochem. 60, 640-644   DOI   ScienceOn
8 Nwaka, S. (1998), Molecular biology of trehalose and trehalases in the yeast, Saccharomyces cerevesiae, Prog. Nuc. Acid Res. Mol. Biol. 58, 197-237
9 Elbein, A. D. (2003), New insights on trehalose: a multifunctional molecule, Glycobiology 13, 17-27   DOI   ScienceOn
10 Elbein, A. D. (1974), The metabolism of $\alpha$, $\alpha$-trehalose, Adv. Carbohydr. Chem. Biochem. 30, 227-256   DOI
11 Nishimoto, T., T. Nakano, H. Chaen, S. Fukuda, T. Sugimoto, M. Kurimoto, and Y. Tsujisaka (1996), Purification and characterization of a thermostable trehalose synthase from Thermus aquaticus, Biosci. Biotech. Biochem. 60, 835-839   DOI
12 Cho, Y. J., S. Koh, D. S. Lee, and H. -J. Shin (2003), Optimization of production of trehalose from maltose using recombinant trehalose synthase from Thermus caldophilus GK24, Korean J. Biotechnol. Bioeng. 18, 8-13
13 Terpe, K. (2003), Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems, Appl. Microbiol. Biotechnol. 60, 523-533   DOI
14 Paiva, C. L. A. and A. D. Panek (1999), Biotechnological applications of the disaccharide trehalose, Biotechnol. Ann. Rev. 2, 293-314
15 Koh, S. (1998), Studies on Trehalose Synthase from Thermus caldophilus GK24, Ph.D. Dissertation, Dept. Agric. Chem., Korea University, Seoul
16 Lee, J. H., K. H. Lee, C. G. Kim, S. Y. Lee, G. J. Kim, Y. H. Park, and S. O. Chung (2005), Cloning and expression of a trehalose synthase from Pseudomonas stutzeri CJ38 in Escherichia coli for the production of trehalose, Appl. Microbiol. Biotechnol. 68, 213219   DOI   ScienceOn
17 Poo, H. Y., J. J. Song, S. P. Hong, Y. H. Choi, S. W. Yun, J. -H. Kim, S. C. Lee, S. G. Lee, and M. H. Sung (2002), Novel high-level constitutive expression system, pHCE vector, for a convenient and cost-effective soluble production of human tumor necrosis factor-$\alpha$, Biotechnol. Lett. 24, 1185-1189   DOI   ScienceOn
18 Saito, K., H. Yamazaki, Y. Ohnishi, S. Fujimoto, E. Takahashi, and S. Horinouchi (1998), Production of trehalose synthase from a basidiomycete, Grifola frondosa, in Esherichia coli, Appl. Microbiol. Biotechnol. 50, 193-198   DOI