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Microcontact Printing of Bacteria Using Hybrid Agarose Gel Stamp  

Shim, Hyun-Woo (Department of Chemical and Biological Engineering, Chungnam National University)
Lee, Ji-Hye (Department of Chemical and Biological Engineering, Chungnam National University)
Lee, Chang-Soo (Department of Chemical and Biological Engineering, Chungnam National University)
Publication Information
KSBB Journal / v.21, no.4, 2006 , pp. 273-278 More about this Journal
Abstract
The noble method of hybrid agarose gel microstamp fabricated by replica molding against PDMS master to make bacteria patterns on agar surface was presented. After the fabricated hybrid agarose gel microstamp was inked with E. coli, we could obtain 2 dimensional bacterial arrays with $50{\mu}m$ circular spots. And the various shaped patterns based on experimental design were easily generated. The analysis of mean fluorescent signal was showed that bacterial pattern have high contrast between spots and background and homogeneity of pattern. Our proposed method solved the problem of wetting and handling with small soft agarose gel microstamp when bacteria were used for ink. The agarose gel stamp provides appropriate environment to inked bacteria, which is essential technology for cell patterning with high retaining viability during the patterning process. This method is reproducible, convenient, rapid, and could be applied to screening system, study of cell-surface interaction, and microbial ecology.
Keywords
Bacterial patterning; microcontact printing; agarose gel;
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1 Kane, R. S., S. Takayama, E. Ostuni, D. E. Ingber, and G. M. Whitesides (1999), Patterning proteins and cells using soft lithography, Biomaterials 20, 2363-2376   DOI   ScienceOn
2 Kim, H. S., Y. M. Bae, Y. K. Kim, B. K. Oh, and J. W. Choi (2006), Antibody layer fabrication for protein chip to detect E. coli O157 : H7, using microcontact printing technique, Journal of Microbiology and Biotechnology 16, 141-144   과학기술학회마을   DOI   ScienceOn
3 Ye, H., Z. Gu, and D. H. Gracias (2006), Kinetics of ultraviolet and plasma surface modification of poly(dimethylsiloxane) probed by sum frequency vibrational spectroscopy, Langmuir 22, 1863-8   DOI   ScienceOn
4 Hui, A. Y., G. Wang, B. Lin, and W. T. Chan (2005), Microwave plasma treatment of polymer surface for irreversible sealing of microfluidic devices, Lab. Chip. 5, 1173-7   DOI   ScienceOn
5 Yu, K., Y. Cong, J. Fu, R. B. Xing, N. Zhao, and Y. C. Han (2004), Patterned self-adaptive polymer brushes by 'grafting to' approach and microcontact printing, Surface Science 572, 490-496   DOI   ScienceOn
6 Trimbach, D. C., M. Al-Hussein, W. H. de Jeu, M. Decre, D. J. Broer, and C. W. Bastiaansen (2004), Hydrophilic elastomers for microcontact printing of polar inks, Langmuir 20, 4738-42   DOI   ScienceOn
7 Kim, J., M. K. Chaudhury, and M. J. Owen (1999), Hydrophobicity loss and recovery of silicone HV insulation, Ieee Transactions on Dielectrics and Electrical Insulation 6, 695-702   DOI   ScienceOn
8 Makamba, H., Y. Y. Hsieh, W. C. Sung, and S. H. Chen (2005), Stable permanently hydrophilic protein-resistant thin-film coatings on poly(dimethylsiloxane) substrates by electrostatic self-assembly and chemical cross-linking, Anal. Chem. 77, 3971-8   DOI   ScienceOn
9 Minas, W., J. E. Bailey, and W. Duetz (2000), Streptomycetes in micro-cultures: growth, production of secondary metabolites, and storage and retrieval in the 96-well format, Antonie Van Leeuwenhoek 78, 297-305   DOI   ScienceOn
10 Wang, B., J. Feng, and C. Gao (2005), Printing biomacromolecules on a bovine serum albumin precursor layer, Macromol. Biosci. 5, 767-74   DOI   ScienceOn
11 Hillborg, H., N. Tomczak, A. Olah, H. Schonherr, and G. J. Vancso (2004), Nanoscale hydrophobic recovery: A chemical force microscopy study of UV/ozone-treated cross-linked poly(dimethylsiloxane), Langmuir 20, 785-94   DOI   ScienceOn
12 Singhvi, R., A. Kumar, G. P. Lopez, G. N. Stephanopoulos, D. I. Wang, G. M. Whitesides, and D. E. Ingber (1994), Engineering cell shape and function, Science 264, 696-8   DOI
13 Park, J. P., S. J. Lee, T. J. Park, K. B. Lee, I. S. Choi, S. Y. Lee, M. G. Kim, and B. H. Chung (2004), Microcontact printing of biotin for selective immobilization of streptavidin-fused proteins and SPR analysis, Biotechnology and Bioprocess Engineering 9, 137-142   DOI   ScienceOn
14 Chen, C. S., M. Mrksich, S. Huang, G. M. Whitesides, and D. E. Ingber (1998), Micropatterned surfaces for control of cell shape, position, and function, Biotechnol. Prog. 14, 356-63   DOI   ScienceOn
15 Lederberg, J. (1989), Replica plating and indirect selection of bacterial mutants: isolation of preadaptive mutants in bacteria by sib selection, Genetics 121, 395-9
16 Duetz, W. A., L. Ruedi, R. Hermann, K. O'Connor, J. Buchs, and B. Witholt (2000), Methods for intense aeration, growth, storage, and replication of bacterial strains in microtiter plates, Appl. Environ. Microbiol. 66, 2641-6   DOI
17 Hall-Stoodley, L., J. W. Costerton, and P. Stoodley (2004), Bacterial biofilms: from the natural environment to infectious diseases, Nat. Rev. Microbiol. 2, 95-108   DOI   ScienceOn
18 Park, J. P., K. B. Lee, S. J. Lee, T. J. Park, M. G. Kim, B. H. Chung, Z. W. Lee, I. S. Choi, and S. Y. Lee (2005), Micropatterning proteins on polyhydroxyalkanoate substrates by using the substrate binding domain as a fusion partner, Biotechnology and Bioengineering 92, 160-165   DOI   ScienceOn
19 Quist, A. P., E. Pavlovic, and S. Oscarsson (2005), Recent advances in microcontact printing, Analytical and Bioanalytical Chemistry 381, 591-600   DOI
20 Park, T. H. and M. L. Shuler (2003), Integration of cell culture and microfabrication technology, Biotechnol. Prog. 19, 243-53   DOI   ScienceOn
21 Balmer, T. E., H. Schmid, R. Stutz, E. Delamarche, B. Michel, N. D. Spencer, and H. Wolf (2005), Diffusion of alkanethiols in PDMS and its implications on microcontact printing (mu CP), Langmuir 21, 622-632   DOI   ScienceOn
22 Thibault, C., V. Le Berre, S. Casimirius, E. Trevisiol, J. Francois, and C. Vieu (2005), Direct microcontact printing of oligonucleotides for biochip applications, J. Nanobiotechnology 3, 7   DOI   ScienceOn
23 Davey, M. E. and A. O'Toole G. (2000), Microbial biofilms: from ecology to molecular genetics, Microbiol. Mol. Biol. Rev. 64, 847-67   DOI
24 Levskaya, A., A. A. Chevalier, J. J. Tabor, Z. B. Simpson, L. A. Lavery, M. Levy, E. A. Davidson, A. Scouras, A. D. Ellington, E. M. Marcotte, and C. A. Voigt (2005), Synthetic biology: engineering Escherichia coli to see light, Nature 438, 441-2   DOI   ScienceOn
25 Lange, S. A., V. Benes, D. P. Kern, J. K. Horber, and A. Bernard (2004), Microcontact printing of DNA molecules, Anal. Chem. 76, 1641-7   DOI   ScienceOn