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Optimization of Submerged Cultivation of Hericium erinaceum  

Jung, Jae-Hyun (Department of Food Science and Technology, Chungju National University)
Lee, Keun-Eok (Department of Biotechnology and Engineerign, Kangwon National University)
Lee, Shin-Young (Department of Biotechnology and Engineerign, Kangwon National University)
Publication Information
KSBB Journal / v.21, no.2, 2006 , pp. 96-102 More about this Journal
Abstract
Recently, it has been known that Hericium erinaceum is a one of the very useful functional materials with great attention in mushroom processing industry. In present study, a liquid culture which was not studied systematically until now, was conducted as a method of cultivation for H. erinaceum, and also examined the characteristics of the liquid culture and conditions of process optimization. A good basal medium was selected through the cultivation of 16 species mushroom media and the optimum condition for medium and cultivation were chosen by response surface method. From these results, the optimum condition of medium for mushroom was 3% glucose, 0.2% yeast extract/peptone(1:1) and 0.1% $KH_2PO_4/MgSO_4$(1:1) and also the optimal culture condition was obtained at inoculum of 13.42%, temperature of $22.3^{\circ}C$ and pH of 5.7. The mycelial dry weight of 9 g/I was obtained under these conditions and this amount was about 1.7 times higher than that which were cultivated in basal medium for 8 days.
Keywords
Hericium erinaceum; mycelium; submerged culture; response surface analysis; optimization;
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1 Kawagishi, H., A. Shimada, R. Shirai, K. Okamoto, F. Ojiima, H. Sakamoto, Y. Ishiguro, and S. Furukawa (1994), Erinacines A, B and C, strong stimulators of nerve growth factor (NGF)-synthesis, from the mycelia of Hericium erinaceum, Tetrahedron Letter 35(10), 1569-1572   DOI   ScienceOn
2 Cho, J. Y. (1996), Liquid cultivation of H. erinaceum mycelium using by food byproducts and compositions of health beverage containing the extract of this mycelium, Korean Patent Application No. 1996-07058
3 Lee, S. Y. and T. S. Kang (1996), Production condition and characterization of exo-polysaccharide production produced by submerged cultivation of Ganoderma lucidum mycelium, Korean J. Appl. Microbiol. Biotechnol. 24(1), 111-118
4 Humfeld, H. (1948), The production of mushroom mycelium (Agaricus campestris) in submerged culture, Science 107, 373-375   DOI
5 Thompson, D. (1982), Response surface experimentation, J. Food Processing and Preservation 6, 155-188   DOI
6 Stamets, P.(1993), Growing Gourmet and Medicinal Mushrooms, p387, Ten Speed Press, Berkeley, CA
7 Ahn, D. K. (1992), Medicinal fungi in Korea, Kor. J. Mycol. 20, 154-165
8 Mizuno, T. (1995), Yamabushitake, Hericium erinaceum: Bioactive substances and medicinal utilization, Food Reviews International 11(1), 173-175   DOI   ScienceOn
9 Statistical Analysis System. Property software (Release 6.03 ed.). SAS Institute Inc., Cary, NC, USA
10 Lee, S. Y. and T. S. Kang (1997), Optimization of antitumor active exo- polysaccharide production through the submerged cultivation of Ganoderma lucidum mycelium, Kor. J. Biotech. Bioeng. 12(2), 139-145
11 Grigansky, A. Ph., E. F. Solomko, and B. Kirchhoff (1999), Mycelial growth of medicinal mushroom Hericium erinaceus (Bull.:Fr.) Pers. in pure culture, International Journals of Medicinal Mushrooms 1(1), 81-87   DOI
12 Baek, G. Y. (2000), Studies on the growth characteristics and functional screening of mycelia and fruiting body of Hericium erinaceum, MS thesis, Dept. of Food Science and Technology, Chungju National University, Chungju
13 Liu. C. Y. (1981), Technique of cultivation of monkey-head mushroom, Edible Fungi No. 4, 33
14 Lee. S. Y. (1996), Characteristics and production of antitumor polysaccharides from mushroom origin in Korean, Biotechnology News 3(2), 95-109
15 Lomberh, M. L., E. F. Solomko, A. S. Buchalo, and B. Kirchhoff (2002), Studies of medicinal mushrooms in submerged cultures, In Mushroom Biology and Mushroom Products, H. P. Graciel, and A. V. Adalberto Eds.; Proceedings of the 4th International Conference of Mushroom Biology and Mushroom Products. Feb. 20-22, 2002, Hotel Villa Bejar, Cuernavaca, Mexico