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Enhanced Lycopene Production in Recombinant Escherichia coli by Random Transposon and NTG Mutagenesis  

Yoon, Sang-Hwal (Department of Food Science and Nutrition, Gyeongsang National University)
Ko, Min-Su (SolGent Co. Ltd.)
Park, Kyoung-Ae (SolGent Co. Ltd.)
Jung, Kyung-Hwa (Amicogen, Inc.)
Shin, Yong-Chul (Department of Microbiology, Gyeongsang National University)
Lee, Young-Mi (Department of Food Science and Nutrition, Gyeongsang National University)
Lee, Sook-Hee (Division of Applied Life Science(BK21), Gyeongsang National University)
Kim, Seon-Won (Department of Food Science and Nutrition, Gyeongsang National University)
Publication Information
KSBB Journal / v.21, no.2, 2006 , pp. 90-95 More about this Journal
Abstract
Escherichia coli harboring pAC-LYCO4 and pDdxs was used for lycopene production. Three wild type strains of E. coli OW1, MG1655, and W3110 were compared with DH5${\alpha}$ used before for lycopene production. Lycopene productivity of E. coli MG1655 was similar to DH5${\alpha}$ and the highest among those wild type strain. Therefore, MG1655 strain was used for random transposon and NTG mutagenesis to increase lycopene productivity. Through transposon mutation, five transposon mutants with increased lycopene productivity were obtained. It was found that genes knocked out by transposon insertion were treB in Tn1 mutant, B2436 in Tn2 mutant, and rfaH in Tn3, 4, and 5 mutants. Lycopene productivity was the highest in Tn4 mutant among the Tn mutants, which was 6-fold and 8-fold higher in lycopene concentration and content, respectively, in comparison with those obtained with wild type strain. NTG4 mutant was acquired with NTG mutation. The highest lycopene productivity of 6 mg/L and 4 mg/g DCW was obtained from the NTG4 mutant when arabinose of 0.013 mM was added for induction of dxs, rate-limiting gene of MEP pathway. The lycopene productivity of NTG4 mutant was increased 18-fold and 12-fold in lycopene concentration and content, respectively when comparing with the wild type strain.
Keywords
Lycopene; random mutation; transposon; NTG; Escherichia coli;
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