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Characterization of PVA Degrading Enzymes from Microbacterium barkeri KCCM 10507 and Paenibacillus amylolyticus KCCM 10508  

Choi Kwang-Keun (Department of Chemical and Biomolecular Engineering, Sogang University)
Kim Sang-Yong (Korea Institute of Industrial Technology)
Lyoo Won-Seok (School of Textiles, Yeungnam University)
Lee Jin-Won (Department of Chemical and Biomolecular Engineering, Sogang University)
Publication Information
KSBB Journal / v.21, no.1, 2006 , pp. 54-58 More about this Journal
Abstract
The purpose of this study is to search the characteristics of PVA degrading enzymes which were obtained from Microbacterium barkeri KCCM 10507 and Paenibacillus amylolyticus KCCM 10508, respectively. As a result of the PVA degrading test using crude enzymes, the activity of SAO (secondary alcohol oxidase) was maximized after 2 or 3 days from start of the test, while the activity of BDH (${\beta}$-diketone hydrolase) was gradually increased during the test. Activities of them were maintained in the presence of PVA, but as PVA was gradually degraded, their activity was decreased. PVA was inoculated again into the media, their activity was revealed. This result indicated that above two different enzymes were closely connected with PVA degradation and PVA was degraded by activity of SAO and BDH. Maximum activity of them was 1.5-1.8 unit for SAO and 1.5-2.0 unit for BDH under $35^{\circ}C$ and pH 7.8-8.8, respectively.
Keywords
${\beta}$-diketone hydrolase; PVA degrading enzymes; secondary alcohol oxidase;
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