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Fibrinolytic Activities and Effects of Gamma-Irradiated on Seeds from Coix lacryma-jobi L. Carthamus tinctorius L. and Malva verticillata L.  

Kwon Su-Jung (Biology Research Center of Industrial Accelerators, Dongshin University)
Lim Chae-Young (Biotechnology Industrialization Center, Dongshin University)
Kim Jae-Sung (Department of Genetic Engineering, Chosun University)
Park Min-Hee (Biology Research Center of Industrial Accelerators, Dongshin University)
Lee Sook-Young (Biology Research Center of Industrial Accelerators, Dongshin University)
Publication Information
KSBB Journal / v.21, no.1, 2006 , pp. 20-27 More about this Journal
Abstract
The fibrinolytic activities of soluble proteins extracted from seeds of Coix lacryma-jobi L., Carthamus tinctorius L. and Malva venicillata L. were studied. Fibrinolytic activity of extract from C. lacryma-jobi L. showed 1.3 times higher than plasmin used as positive control. The fibrinolytic enzyme was confirmed and extracted directly from seed of C. lacryma-jobi L. by a fibrin zymography. The protein was composed of a single polypeptide and its apparent molecular weight was found to be 7.8 kDa, as judged by SDS-polyacrylamide gel electrophoresis. The effect of temperature for the proteolytic enzyme activity were stabilized above $50^{\circ}C$ and then dramatically decreased. Also, the enzyme activity was clearly inhibited by APMSF, PMSF and TPCK, suggesting that it is a member of the chymotrypsin-like serine pretense. In addition, effects of gamma-irradiated on seed of each plants were revealed that 8 Gy and 64 Gy were higher than others. This result shown that gamma-irradiation of seeds were capable to increase the fibrinolytic activity. All these results suggest the pretense is a fibrinolytic enzyme belong to a family of chymotrypsin-like serine pretense.
Keywords
Coix lacryma-jobi L.; Carthamus tinctorius L.; Malva verticillata L.; fibrinolytic enzyme; gamma-irradiation;
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1 Davie, E. W., K. Fugikawa, and W. Kisiel (1991), The coagulation cascade: initiation, maintenance, and regulation, Biochemistry 30, 10363-10370   DOI   ScienceOn
2 Mullertz, S. and M. Lassen (1953), An activator system in blood indispensable for formation of plasmin by streptokinas,. Proc. Soc. Exp. Biol. Med. 82, 264
3 D'Costa, S. S. and M. D. P. Boyle (1998), Interaction of a group A Streptococcus within human plasma results in assembly of a surface plasminogen activator that contributes to accupancy of surface plasmin-binding structures, Microbial Pathogenesis 24, 341-349   DOI   ScienceOn
4 Sumi, H., M. Seiki, N, Morimoto, H. Tsushima, M. Maruyama, and H. Mihara (1985), Plasma fibrinolysis after intraduodenal administration of urokinase in rats, Enzyme. 33, 121-127   DOI
5 Park, H-J. and S-D. Park (1998), Purification and chrarcteristics of fibrinolytic enzyme having molecular weight 45,000 dalton from Holotrachia extract, J. Herbology 13(1), 119-137
6 Ouyang, C. and T. F. Huang (1976), Purification and properties of the fibrinolytic principle of Agkistrodon acutus venom, Biochimica et Biochemica Acta. 439, 146-153   DOI   ScienceOn
7 Datta, G., A. Dong, J. Witt, and A. T. Tu (1995), Biochemical characterization od basilase, a fibrinolytic enzyme from Crotalus basilicus basilicus, Achieves Biochemistry and Biophysics 317, 365-373   DOI   ScienceOn
8 Guo, Y. W., T. Y. Chang, K-T. Lin, H-W. Liu, K-C. Shih, and S-H. Cheng (2001), Cloning and functional expression of the mucrosobin protein, a $\beta$-fibrinogenase of Trimeresurus mucrosquaqmatus (Taiwan Habu), Protein Expression and Purification 23, 483-490   DOI   ScienceOn
9 Fan, Q., C. Wu, L. Li, R. Fan, C. Wu, Q. Hou, and R. He (2001) Some features of intestinal absorption of intact fibrinolytic enzyme III-1 from Lumbricus rubellus, Biochimica et Biochemica Acta. 1526, 286-292   DOI   ScienceOn
10 Mihara, H., N. Nakajima, and H. Sumi (1993), Characterization of potent fibrinolytic enzymes in earthworm, Lumbricus rubellus, Biosci. Biotech. Biochem. 57, 1730
11 Kim, Y. T., W. K. Kim, and H. S. Oh (1995), Screening and Identification of the Fibrinolytic Bacterial Strain from ChungKooK-jang, Kor. J. Microbiol. Biotechnol. 2(3), 1-5
12 Matsubara, K., H. Sumi, K. Hori, and K. Miyazawa (1998), Purification and characterization of two fibrinolytic enzymes from a marine green alga, Codium intricatum, Comparative Biochemistry and Physiology 119B(1), 177-181
13 Matsubara, K., K. Hori, Y. Matsuura, and K, Miyazawa (1999), A fibrinolytic enzyme from a marine green alga, Codium latum, Phytochemistry 52, 993-999   DOI   ScienceOn
14 Kim, J. H. and Y. S. Kim (1998), Purification and Characterization of Fibrinolytic enzyme from Armillariella mellea, The korean Society of Mycology 26(4), 583-588
15 Sanchez, E. F., C. I. Santos, A. Magalhaes, C. R. Diniz, S. Figueiredo, J. Giloy, and M. Richardson (2000), Isolation of a proteinase with plasminogen-activating activity from Lachesis muta muta (bushmaster) snake venom, Achives of Biochemistry and Biophysics. 378(1), 131-141   DOI   ScienceOn
16 Nakajima, N., N. Taya, and H. Sumi (1993), Potent fibrinolytic enzyme from the lysate of Katsuwonus pelamis digestive tract (shiokara): purification and characterization, Biosci. Biotech. Biochem. 57(9), 1604-1605   DOI   ScienceOn
17 Sumi, H., N. Nakajima, and C. Yatagai (1995), A unique strong fibrinolytic enzyme (katsuwokinase) in skipjack Shiokara, a Japanes traditonalmfermented food, Comp. Biochem. Physiol B. 112(3), 543-547   DOI   ScienceOn
18 Ahmed, N. K., K. D. Tennant, F. S. Markland, and J. P. Lacz (1990), Biochemical characteristics of fibrolase, a fibrinolytic protease from snake venom, Haemostasis 20, 147-154
19 Hahn, B. S., S. Y. Cho, M. Y. Ahn, and Y. S. Kim (2001), Purification and characterization of plasmin-like protease from Tenodera sinensis (Chinese mantis), Insect Biochemistry and molecular biology 31, 573-581   DOI   ScienceOn
20 Hrzenjak, T., M. Popovic, T. Bozic, M. Grdisa, D. Kobrehel, and L. Tiska-Rudman (1998), Fibrinolytic and anticoagulative activities from the earthworm Eisenia foetida,. Comp. Biochem. Physiol B. 119, 825-832   DOI   ScienceOn
21 Sugiki, M., Yoshida, E., Anai, K., and M. Maruyama (1998), Activation of single-chain urokinase-type plasminogen activator by a hemorrhagic metalloproteinase, jararafibrase I, in Bothrops Jararaca venom, Toxicon. 36, 993-1000   DOI   ScienceOn
22 Matsubara, K., K. Hori, Y. Matsuura, and K. Miyazawa (2000), Purification and characterization of a fibrinolytic enzyme and identification of fibrinogen clotting enzyme in a marine green alga, Codium divaricatum, Comparative Biochemistry and Physiology 125, 137-143   DOI   ScienceOn
23 Jin, Y., Q. M. Lu, J. F. Wei, D. S. Li, W. Y. Wang, and Y. L. Xiong (2001), Purification and characterization of jerdofibrase, a serine protease from the venom of Trimeresurus jerdonii snake, Toxicon. 39, 1203-1210   DOI   ScienceOn
24 Jean, O-H., W-J. Moon, and D-S. Kim (1995), An anticoagulant/fibrinolytic protease from Lumbricus rubellus, J. Biochem. Mol. Biol. 28(2), 138-142
25 Reed, G. L., L. F. Lin, B. Parhaml-Seren, and P. Kussie (1995), Identification of plasminogen binding. region in streptokinase that is necessary for the creation of streptokinase plasminogen activator complex, Biochemistry 34, 10266-10271   DOI   ScienceOn
26 Bajwa, S. S., H. Kirakossian, K. N. N. Reddy, and F. S. Markland (1982), Thrombin-like and fibrinolytic enzymes in the venoms from the gaboon viper (Bitis gabonica), eastern cottomnouth moccasin (Agkistrodon p. piscivorus) and southern copperhead (Agkistrodon c. contortrix) snakes, Toxicon. 20, 427-432   DOI   ScienceOn
27 Ramirez, M. S., E. E. Sanchez, C. Garcia-Prieto, J. C. Perez, G. R. Chapa, M. R. McKeller, R. Ramirez, and Y, D. Anda (1999), Screening for fibrinolytic activity in eight viperid venoms, Com. Biochem. Physiol. 124(C), 91-98
28 Hamouda, B. M. H. and M. Ammar (1984), Demonstration of plasminogen activators in the saliva of a predatory insect of the family of Reduviidae, Arch. Inst. Pasteur. Tunis. 61, 73-95
29 Kim, J. H. (2000), Purification and Characterization of Fibrinolytic enzyme from Tricholoma saponaceum, The korean Society of Mycology 28(1), 60-65
30 Dauod, E., A. Tu, and M. F. el-Asmar (1986), Isolation and characterization of an anticoagulant proteinase cerastase F-4 from Cerastes cerastes (Egyptian sand viper) venom, Thromb. Res. 42, 55-62   DOI   ScienceOn
31 Sumi, H., H. Hamada, H. Tsushima, H. Mihara, H., and H. A. Muraki (1987), A nobel fibrinolytic enzyme (nattokinase) in the vegetable cheese Natto: a typical and popular soybean food in the Japanese diet, Experientia. 43, 1110-1111   DOI   ScienceOn
32 Kim, S. H. (1998), New trends of studying on potential activities of Doen-jang. Korea Soybean Diges. 15(1), 8-15
33 Nakajima, N., H. Mihara, and H. Sumi (1993), Characterization of potent fibrinolytic enzymes in earthwarm, Lumbricus rubellus, Biosci. Biotech. Biochem. 57(10), 1726-1730   DOI   ScienceOn
34 Siigur, E. and J. Siigur (1991), Purification and characterization of lebetase, a fibrinolytic enzyme from Vipera lebetina (snake) venom, Biochim. Biophys. Acta. 1074, 223-229   DOI   ScienceOn
35 Chiou, S. H., C. C. Hung, and K. F. Huang (1992), Characterization of a protease with alpha- and beta-fibrinogenase activity from the western diamondback rattlesnake, Crotalus atrox, Biochim. Biophys. Res. Commun. 187, 389-396   DOI   ScienceOn
36 Laemmli, U. K. (1970), Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227, 680-685   DOI   ScienceOn
37 Markland, F. S. (1998), Snake venom and hemostatic system, Toxicon. 36(12), 1749-1800   DOI   ScienceOn
38 Hellmann, K. and R. I. Hawkins (1964), Anticoagulant and fibrinolytic activities from Rhodnius prolixus, Stal. Nature 201, 1008-1009   DOI   ScienceOn
39 Zhang, Y., A. Wisner, Y. Xiong, and C. Bon (1995), A novel plasminogen activater from snake venom, J. Biol. Chem. 270, 10246-10255   DOI
40 Trummal, K., H. Vija, J. Subbi, and J. Siigur (2000), MALDI-TOF mass spectrometry analysis of substrate specificity of lebetase, a direct-acting fibrinolytic metalloproteinase from Vipera lebetina snake venom, Biochimica et Biochemica Acta. 1476, 331-336   DOI   ScienceOn
41 Patton, L. M., D. Pretzer, B. S. Schulteis, K. D. Saggart, K. D. Tennant, and N. K. Ahmed (1993), J. Biochem. Biophysi. Met. 27(1), 11-23   DOI   ScienceOn
42 Swenson, S., L. R. Bush, and F. S. Markland (2000), Chimeric derivative of fibrolase, a fibrinolytic enzyme from southern copperhead venom, processes inhibitory activity on platelet aggregation, Achieves Biochemistry and Biophysics 384(2), 227-237   DOI   ScienceOn
43 Chung, K. H. and D. S. Kim (1992), Fibrinolytic and Cogulation Activities of Korean Snake Venoms, Korean Biochem. J. 25, 696-701
44 Hahn, B. S., I. M. Chang, and Y. S. Kim (1995), Purification and characterization of picivorase I and II, the fibrinolytic enzymes from eastern cottomnouth moccasin venom (Agkistrodon piscivorus piscivorus), Toxicon. 33(7), 929-941   DOI   ScienceOn
45 Astrup, T. and S. Mullertz (1952), The fibrin agarose plate method for estimating fibrinolytic activity, Arch. Biochem. Biophys. 40, 346-351   DOI   ScienceOn
46 Fujita, M., K. NoMura, K. Hong, Y. Ito, A. Asada, and S. Nishimura (1993), Purification and characterization of a strong fibrinolytic enzyme (nattokinase) in the vegetable cheese natto, a popular soybean fermented food in Japan, Biochem. Biophys. Res. Commun. 197, 1340-1347   DOI   ScienceOn
47 Amarant, T. W. Burkltart, H. D. LeVine, C. L. Arocha-Pinango, and I. Parikh (1991), Isolation and complete amino acid sequence of two fibrinolytic proteases from the toxic Saturnid caterpillar Lonomia achelous, Biochim. Biophys. Acta. 1079, 214-221   DOI   ScienceOn
48 Koh, Y. S., K-H. Chung, and D. S. Kim (2001), Bionchemical characterization of a thrombin-like enzyme and a fibrinolytic serine protease from snake (Agkistrodon saxatilis) venom, Toxicon. 39, 555-560   DOI   ScienceOn
49 Chudzinski-Tavassi, A. M., E. M. Kelen, A. P. Paula-Rosa, S. Loyau, C. A. Sampaio, C. Bon, and E. Angles-Cano (1998), Fibrino(geno)lytic properties of purified hementerin, a metallo-proteinase from the leech Haementeria depressa, Thromb. Haemost. 80, 155-160   DOI
50 Matsushima, A., K. Shioya, M. Kobayashi, Y. Kodera, and Y. Inada (1993), Activation of fibrinolysis with the protease from Dermatophagoides farinae, Thromb. Haemost. 70, 545
51 Xiuxia, L., C. Jiashu, Z. Yingna, Q. Pengxin, and Y. Grangmei (2001), Purification and biochemical characterization of F IIa a fibrinolytic enzyme from Agkistrodon acutusvenom, Toxicon. 39, 1133-1139   DOI   ScienceOn
52 Mihara, H., H. Sumi, T. Yoneta, H. Mizumoto, R. Ikeda, M. Seiki, and M. Maruyama (1991), A novel fibrinolytic enzyme extracted from the earthworm, Lumbricus rebellus, Japanese journal of Physiology 41, 461   DOI   ScienceOn
53 Bradford, M. M. (1976), A Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Analytical Biochemistry 72, 248-254   DOI   ScienceOn
54 Holden, R. W. (1990), Plasminogen activators: pharmacology and theraphy, Radiology 174, 993-1001   DOI
55 Siigur, J., M. Samuel, K. Tonismagi, J. Subbi, E. Siigur, and A. T. Tu (1998), Biochemical characterization of lebetase, a direct-acting fibrinolytic enzyme from Vipera lebetina snake venom, Thrombosis research 90, 39-49   DOI   ScienceOn
56 Kim, H. K., G. T. Kim, D. K. Kim, W. A. Choi, S. H. Park, Y. K. Jeong, and I. S. Kong (1997), Purification and Characterization of a novel Fibrinolytic Enzyme from Bacillus sp. KA38 Originated from Fermented Fish, J. Fermentation and Bioengineering 84(4), 307-312   DOI   ScienceOn