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Fibrinolytic Activities and Effects of Gamma-Irradiated on Seeds from Coix lacryma-jobi L. Carthamus tinctorius L. and Malva verticillata L.  

Kwon Su-Jung (Biology Research Center of Industrial Accelerators, Dongshin University)
Lim Chae-Young (Biotechnology Industrialization Center, Dongshin University)
Kim Jae-Sung (Department of Genetic Engineering, Chosun University)
Park Min-Hee (Biology Research Center of Industrial Accelerators, Dongshin University)
Lee Sook-Young (Biology Research Center of Industrial Accelerators, Dongshin University)
Publication Information
KSBB Journal / v.21, no.1, 2006 , pp. 20-27 More about this Journal
Abstract
The fibrinolytic activities of soluble proteins extracted from seeds of Coix lacryma-jobi L., Carthamus tinctorius L. and Malva venicillata L. were studied. Fibrinolytic activity of extract from C. lacryma-jobi L. showed 1.3 times higher than plasmin used as positive control. The fibrinolytic enzyme was confirmed and extracted directly from seed of C. lacryma-jobi L. by a fibrin zymography. The protein was composed of a single polypeptide and its apparent molecular weight was found to be 7.8 kDa, as judged by SDS-polyacrylamide gel electrophoresis. The effect of temperature for the proteolytic enzyme activity were stabilized above $50^{\circ}C$ and then dramatically decreased. Also, the enzyme activity was clearly inhibited by APMSF, PMSF and TPCK, suggesting that it is a member of the chymotrypsin-like serine pretense. In addition, effects of gamma-irradiated on seed of each plants were revealed that 8 Gy and 64 Gy were higher than others. This result shown that gamma-irradiation of seeds were capable to increase the fibrinolytic activity. All these results suggest the pretense is a fibrinolytic enzyme belong to a family of chymotrypsin-like serine pretense.
Keywords
Coix lacryma-jobi L.; Carthamus tinctorius L.; Malva verticillata L.; fibrinolytic enzyme; gamma-irradiation;
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