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Characterization of Bacillus licheniformis as a Probiotic  

Kim Jin-Wook (Department of Bioscience and Biotechnology, College of Engineering, Silla University)
Jun Kyoung-Dong (Research and Development Center, Binex Co. Ltd)
Kang Jae-Seon (Department of Pharmaceutical Engineering, Inje University)
Jang Jung-Su (Research and Development Center, Binex Co. Ltd)
Ha Bae-Jin (Department of Bioscience and Biotechnology, College of Engineering, Silla University)
Lee Jae-Hwa (Department of Bioscience and Biotechnology, College of Engineering, Silla University)
Publication Information
KSBB Journal / v.20, no.5, 2005 , pp. 359-362 More about this Journal
Abstract
Bacillus licheniformis was an useful material for improvement of fecal microflora. The stability of Bacillus licheniformis in low pH (pH 2, 3, 4, 6, 7), artificial bile acid, high concentration of salt (0, 5, 10, 20, $30\%$), and ethanol (0, 4, 8, 16, $32\%$) was investigated in this study. The viability of Bacillus licheniformis was stable for pH 3, 4, 6, 7 Final viability of Bacillus licheniformis was confirmed below $25\%$, in the bile acid. In high concentration of salt, Bacillus licheniformis showed optimal viability in $10\%$ of salt. The viability of Bacillus licheniformis was over $95\%$ in condition of ethanol concentration $4\%,\;8\%,16\%,\;32\%$ contrast with uncontained ethanol at $37^{\circ}C$ for 4h. It was verified that Bacillus licheniformis was stable for pH 4, 6, 7, high concentration of salt and ethanol but was unstable in pH 2 and bile acid.
Keywords
Bacillus licheniformis; probiotic; fecal microflora;
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1 Jung, K. D., H. J. Kim, K. H. Lee, H. D. Paik, and J. S. Kang (2002), Characterization of Bacillus polyfermenticus SCD. as a Probiotics, Kor. J. Microbiol. Biotechnol. 30, 359-366
2 Goossens, D., D. Jonkers, M. Russel, A. thijs, A. van den Bogaard, E. Stobberingh, and R. Stockbrugger (2005), Survival of the probiotics, L. plantarum 299v and its effect on the faecal bacterial flora, with and without gastric acid inhibition, Digest. Liver Dis. 37, 44-50   DOI   ScienceOn
3 Conway, P. L., S. L. Gorbach, and B. R. Goldin (1987), Survival of lactic acid bacteria in the human stomach and adhesion to interstinal cells, J. Dairy Sci. 70, 1-12   DOI   ScienceOn
4 Giannella, R. A., S. A Broitman, and N. Zamcheck (1972), Gastric acid barrier to ingested microorganisms in man: Studies in vivo and in vitro, Gut. 13, 251-256   DOI   ScienceOn
5 Koo, S. M., Y. H. Cho, C. S. Huh, Y. J. Beak, and J. Y. Park (2001), Improvement of the stability of Lactobacillus casei YIT 9018 by microencapsulation using alginate and chitosan, J. Microbiol. Biotechnol. 11, 376-383
6 Bongaerts, G., R. Severijnen, and H. Tinnnennan (2005), Effect of antibiotics, prebiotics and probiotics in treatment for hepatic encephalopathy, Med Hypotheses 64, 64-68   DOI   ScienceOn
7 Paik, H. D., M. Y. Jung, H. Y. Jung, W. S. Kim, and K. T. Kim (2002), Characterization of Bacillus polyfermenticus SCD for Oral Bacteriotherapy of Gastrointestinal Disorders, Korean. J. Food Sci. Technol. 34, 73-78
8 Fuller, R. (1989), Probiotics in man and animals, J. Appl. Bacteriol. 66, 365-378   DOI
9 Berrada, N., J. F. Lemeland, G. Laroche, P. Thouvenot, and M. Piaia (1991), Bifidobacterium from fermented milks: Survivals during gastric transit, J. Dairy Sci. 74, 409-413   DOI   ScienceOn
10 Jun, K. D. (1998), Microbiological identification, antimicrobial Activity and enhanced production of bisroot strain, M. S. Thesis, Kyungnam University
11 Campbell, G. L. and M. R. Bedford (1992), Enzyme applications for monogastric feeds, A reivew. can. J. Anim. Sci. 72, 449-466   DOI
12 Tannock, G. W. (1997), Probiotic properties of lactic acid bacteria: plenty of scope for fundamental R&D, Trends. Biotechnol. 15, 270-274   DOI   PUBMED   ScienceOn
13 Gilliland, S. E., T. E. Staley, and L. J. Bush (1984), Importance of bile tolerance of Lactobacillus acidophilus used as a dietary adjunct, J. Dairy Sci. 67, 3045-3051   DOI   ScienceOn