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Medium Composition of Enterobacter cloacae YJ-1 for Maximizing Hydrogen Production  

Lee Ki-Seok (Department of Environmental Engineering, Chonnam National University)
Kang Chang-Min (Department of Environmental Engineering, Chodang National University)
Chung Seon-Yong (Department of Environmental Engineering, Chonnam National University)
Publication Information
KSBB Journal / v.20, no.5, 2005 , pp. 350-354 More about this Journal
Abstract
In order to maximize hydrogen production by Enterobacter cloacae YJ-1, anaerobic hydrogen producing bacteria, the medium composition was optimized. Glucose was better than other carbon sources in hydrogen production and its production was 975.4 mL/L at $2\%$ (w/v) for 48 h. Organic nitrogen sources were more effective than inorganic nitrogen sources and also yeast extract among organic nitrogens was the most effective in hydrogen production. Among metal ions, $Na_2MoO_4$ was most effective, and its production was 1753.3mL/L at $0.04\%$ (w/v). Addition of amino acid was very effective with compare to another components of medium, and cystein was most effective among them. Under the optimum medium obtained in batch culture, semi-batch culture in order to produce continuous hydrogen was run. The highest hydrogen production was earned at $3\%$(w/v) of glucose and the amount was 2215.4 mL/L.
Keywords
Hydrogen production; Enterobacter cloacae YJ-1; medium composition; amino acid; semi-continuous culture;
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