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The Effect of Weight Ratio of Carbon Source to Nitrogen Source on toe Growth and the Composition of Fatty Acid of Thraustochytrium aureum ATCC 34304  

Kim Won-Ho (Department of Biological and Chemical Engineering, College of Engineering, Inha University)
Park Seung-Hye (Department of Biological and Chemical Engineering, College of Engineering, Inha University)
Song Sang-Kyu (Department of Biological and Chemical Engineering, College of Engineering, Inha University)
Bae Kyung-Dong (Vaccine Research Institute, GreenCross Vaccine Corp.)
Hur Byung-Ki (Department of Biological and Chemical Engineering, College of Engineering, Inha University)
Publication Information
KSBB Journal / v.20, no.4, 2005 , pp. 266-270 More about this Journal
Abstract
The effect of the weight ratio of carbon source to nitrogen source on the growth and the composition change of fatty acids of Thraustochytrium aureum ATCC 34304 was investigated. The cell concentration of 5 days' culture increased and then decreased according to the increase of C/N, The ratioes for the maximum biomass were unique respectively and distributed between 1 and 4 with the initial sugar concentration in the range of 5 g/L to 25 g/L. The biomass yield, $Y_{x/s}$ decreased along with the increase of C/N, but maintained a constant value 0.35 between 10 and 20. The composition of myristic acid $(C_{14:0})$, one component of the lipid synthesized by T. aureum, was not affected with the change of C/N, but the composition of palmitic acid $(C_{16:0})$ was around $20\%$ below 4 of C/N and decreased to $15\%$ according to C/N about 4. The compositions of oleic acid $(C_{18:1})$ and linoleic acid $(C_{18:2})$ increased from 0 to $20\%\;and\;7\%$ respectively. The composition of $\gamma-linoleic$ acid $(C_{18:3})$, however, reduced from $5\%\;to\;2\%$. EPA $(C_{20:5})$ and DPA $(C_{22:5})$ showed a tendency of reduction in the weight composition according to the increase of C/N, but DHA $(C_{22:6})$ had a trend maintaining an approximately constant value, around $40\%$, irrespective of the change of C/N.
Keywords
Thraustochytrium aureum; polyunsaturated fatty acids; carbon source; nitrogen source;
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