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Effect of Functional Monomer on Retention Factor of Chiral Racemate  

Jin, Yin-Zhe (Center for Advanced Bioseparation Technology and Department of Chemical Engineering, Inha University)
Row, Kyung-Ho (Center for Advanced Bioseparation Technology and Department of Chemical Engineering, Inha University)
Publication Information
KSBB Journal / v.20, no.4, 2005 , pp. 260-265 More about this Journal
Abstract
In this work, molecular imprinted polymers (MIPs) using the template of the N-CBZ (carbobenzyloxy)-L-phenylalanine, MAA and 4-VPY as a monomer, EGDMA as a crosslinker and AIBN as an initiator were considered. The prepared polymer particles $(Ca.\;25-35\;{\mu}m)$ were packed into a chromatographic column $(3.9\;\times\;150\;mm)$. The chromatographic characteristics of the retention on the MIP were experimented with acetonitrile as a mobile phase at the flow rate of mobile phase, 0.5 ml/min. The retention factors and resolutions of chiral racemate of the N-CBZ-D, L-phenylalanine were measured. The results showed that the retention factor and resolution by the two co-monomer imprinting polymer were higher than the single monomer imprinting polymers, which indicated an increase in the affinity of the MIP with the sample as a result of the cooperation effect of the binding sites.
Keywords
Molecular imprinted polymer; co-monomer; hydrogen binding; chiral racemate; retention factor;
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