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Batch Production of Chiral Epichlorohydrin by Enantioselective Hydrolysis Reaction using Rhodosporidium toruloides  

이은열 (경성대학교 공과대학 식품공학과)
이재화 (신라대학교 공과대학 생명공학과)
Publication Information
KSBB Journal / v.19, no.1, 2004 , pp. 38-41 More about this Journal
Abstract
Enantioselective hydrolysis for the producing chiral epichlorohydrin from its racemic substrate was investigated using epoxide hydrolase activity of Rhodosporidium toruloides SJ-4. The effects of reaction parameters including pH, temperature, initial substrate concentration on initial hydrolysis rate and enantioselectivity were analyzed and optimized. The addition of detergent, Tween 20, enhanced the hydrolysis rate and enantioselectivity. Chiral (R)-epichlorohydrin with high optical purity (>99% ee) and yield of 25% (theoretically 50% maximum yield) was obtained from its racemate of 20 mM.
Keywords
Enantioselective hydrolysis; epichlorohydrin; epoxide hydrolase; Rhodosporidium toruloides;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
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