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Separation of Glabridin from Licorice by RP-HPLC  

정용안 (초정밀생물분리기술연구센터, 인하대학교 화학공학과)
이광진 (초정밀생물분리기술연구센터, 인하대학교 화학공학과)
권문주 (초정밀생물분리기술연구센터, 인하대학교 화학공학과)
노경호 (초정밀생물분리기술연구센터, 인하대학교 화학공학과)
Publication Information
KSBB Journal / v.18, no.5, 2003 , pp. 408-411 More about this Journal
Abstract
By reversed-phase high-performance liquid chromatography, the extraction and separation of glabridin by from licoricce root was performed in this work. The column efficiencies and resolutions of glabridin were investigated with mobile phase composition on the reversed-phase chromatographic system. The glabridin collected from licorice root was identified by LC/MS. The mobile phase used to extract glabridin were composed of ethanol, methanol, acetone, and ethyl acetate. For one-hour ultrasonic extraction with solvent of ethyl acetate, the favorable content of glabridin was obtained as 1.26g/kg. The glabridin was well separated in the mobile phase composition of 50/50 vol. % (acetonitrile/water).
Keywords
HPLC; glabridin; licoricce; extraction; separation;
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