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Effects of Codonopsis lanceolata Extracts on bFGF-induced Angiogenesis in vitro  

소준노 (우석대학교 생명공학부 우석대학교 식품영양 및 공학과)
김종화 (우석대학교 생명공학부 우석대학교 식품영양 및 공학과)
Publication Information
KSBB Journal / v.18, no.1, 2003 , pp. 25-29 More about this Journal
Abstract
In this study, we examined the effects of the methanolic extract(CL-ex) of Codonopsis lanceolata on the angiogenesis stimulated with basic fibroblast growth factor(bFGF) in vitro, using porcine pulmonary arterial endothelial cells(PPAECs). In addition, we investigated the endothelial functions involved in angiogenesis, such as proliferation, migration and secretion of matrix metalloproteinases(MMPs), using human umbilical vein endothelial cells(HUVECS). CL-ex inhibited FGF-induced sprout formation in vitro at concentrations of 0.1-100 ug/ml. Although CL-ex did not affect the proliferation of endothelial cells, CL-ex strongly inhibited the FGF-induced migration of HUVECS at concentrations of 0.1-1 ug/ml; the degree of inhibition of endothelial cells by C-ex was 49.4% at 0.1 ug/ml and 71.9 % at 1.0 ug/ml. Moreover, CL-ex inhibited the secretion of MMPs from HUVECS stimulated with FGF. Therefore, the inhibitory effect of CL-ex on angiogenesis in vitro could be explained by the inhibition of endothelial cell migration. From these results, we suggest that Codonopsis lanceolata is a useful herb for the development of therapeutics or preventive food factors for angiogenesis related diseases, such as tumors.
Keywords
Codonopsis lanceolata; bFGF; angiogenesis; MMPs;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
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