Browse > Article

Efficient Purification Of Fused Ferritin[$F_{H}+F_{L}$] using Silica Powder and Gel Filtration Chromatography  

허윤석 (충남대학교 공과대학 화학공학과)
김인호 (충남대학교 공과대학 화학공학과)
Publication Information
KSBB Journal / v.17, no.4, 2002 , pp. 365-369 More about this Journal
Abstract
An iron-storage protein, ferritin is a spherical shell consisting of 24 H-and L-chain subunits. Soluble form of fused($F_{H}+F_{L}$ chain) ferritin was separated from disrupted recombinant E. coii cells, followed by silica powder adsorption. Ferritin was recovered from silica-poweder by distilled water, which was applied to gel filtration chromatography(GFC). Collected ferritin fractions from the GFC were assayed via iron-uptake and its molecular weight determined using GF-HPLC. Fused ferritin showed a higher activity than the M- or L- chain ferritin by two times.
Keywords
Ferritin; purification; gel filtration chromatography;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Seo, H. Y., E. S. Jeon, Y. J. Chung, and K. S. Kim (2002), Heterologous Expression of Human Ferritin H-Chain and L-Chain Genes in Saccharomyces cerevisiae, Kor. J. Biotechnoi. Bioeng. 17(2), 162-168   과학기술학회마을
2 Theil, E. C (1987), Ferritin: Structure, Gene Regulation and Cellular Function in Animals, Plants and Microorganisims, Annu. Rev. Biochem. 56, 289-315   DOI   ScienceOn
3 Geetha, C. and V. Deshpande (1999), Purification and Characterization of Fish Liver Ferritins, Biochem. Physiol. 123, 285-294   DOI   ScienceOn
4 Kim, C. W (1981), Isolation and Characterization of Ferritin from Rat Intestinal Mucosa, J. Biochem. 14(4), 303-314   DOI
5 Laulhere, J. P., A. M. Lescure, and J. F. Briat (1988), Purification and Characterization of Ferritins from Maize Pea, and Soya Bean Seeds. Distribution in various Pea Organs, J. Bioi. Chem. 263, 10289-10294
6 Barcelo, F., F. Miralles, and C. O. Arean (1997), Purification and Characterization of Ferritin from Alfalfa Seeds, J. lnorg. Biochem. 66, 23-27   DOI   ScienceOn
7 Levi, S., A. Salfeld, F. Franceschinelli, A. Cozzi, M. H. Domer, and P. Arosio (1989), Expression and structural and functional properties of human ferritin L-chain from Escherichia coli, Biochem. 28, 0162-0134   DOI
8 Kim, K. S., H. R Mun, and J. H. Lee (1999), Iron Cores of Tadpole Ferritin: Native, Reconstituted and Recombinant H-Chain Ferritins, lnorg. Chim. Acta. 298,107-111   DOI   ScienceOn
9 Hossein, A. T., J. H. Aron, and R.T. Andrew (1999),Ferritin Mutants of Escherichia coli are Growth Impaired and fur Mutants are Iron Deficient , Appl. J. Bacterial. 181(5), 1415-1428
10 Kim, R A., S. G. Lee, and C. Y. Yun (1996), Purification of Ferritin of Larval Haemolymph from Fall Webworm, Hyphantria cunea, J. Entomol. 26(2), 135-141
11 Kim, S. W., Y. H. Kim, and J. W. Lee (2001), Thermal Stability of Human Ferritin: Concentration Dependence and Enhanced Stability of an N-Terminal Fusion Mutant, Biochem. Biophys. Res. Comm. 289, 125-129   DOI   ScienceOn
12 Sczekan, S. R and J. G. Joshi (1987), Isolation and Characterization of Ferritin from Soyabeans (Glycine max), J. Bioi. Chem. 262, 13780-13788
13 Arosio, P., S. Levi, E. Gabri, S. Stefanini, A. A. Finazzi, and Chinancone (1984), Properties of Ferritin from the Earthworm Octolasium complanatum, J. Cell. Sci. 58, 225-240
14 Geetha, C. and V. Deshpande (1999), Purification and Characterization of Fish Liver Ferritins, Biochem. Physiol. 123, 285-294   DOI   ScienceOn
15 Santambrogio, P., S. Levi, A. Cozzi, E. Rovida, A. Albertini, and P. Arosio(1988), Production and Characterization of Recombinant Heteropolymers of Human Ferritin Hand L Chains, J. Bioi. Chem. 263, 18086-18092
16 Laulhere, J. P., A. M. Laboure, and J. F. Briat (1989), Mechanism of the Transition from Plant Ferritin to Phytosiderin, J. Bioi. Chem. 264, 3629-3635
17 Hudson, A. J., S. C. Andrews, C. Hawkins, J. M. Williams, M. Izuhara, F. C. Meldrum, S. Mann, P. M. Harrison, and J. R Guest (1993), Overproduction, Purification and Characterization of the Escherichia coli Ferritin, Eur. J. Biochem. 218, 985-995   DOI   ScienceOn
18 Arosio, P., T. G. Adelman, and J. W. Drysdale(1978), On Ferritin Heterogeneity, Further Evidence for Heteropolymers, J. Bioi. Chem. 253, 4451-4458