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Simple/Rapid Method for RNA Preparation from Lactobacillus spp.  

소재성 (인하대학교 생물공학과, 초정밀 생물분리기술연구센터)
오은택 (인하대학교 생물공학과, 초정밀 생물분리기술연구센터)
최민지 (인하대학교 생물공학과, 초정밀 생물분리기술연구센터)
윤현식 (인하대학교 생물공학과, 초정밀 생물분리기술연구센터)
Publication Information
KSBB Journal / v.17, no.3, 2002 , pp. 311-313 More about this Journal
Abstract
Lactobacillus spry. are Gram-positive bacteria playing important roles in human health. In this study, we successfully isolated the total RNA from the cells broken by glass beads using hot phenol method. Moreover, we were able to omit lysozyme and proteinase K treatment by using glass beads to break cell more efficiently. This method was more rapid and simple when compared to the previous one. Prepared RNA can be used for the transcriptional analysis of Lactobacillus spp.
Keywords
Lactobacillus spp.; RNA preparation;
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Times Cited By KSCI : 2  (Citation Analysis)
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