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Selection and Characterization of a High Erythritol Producing Mutant of Moniliella suaveolens var. nigra  

박홍우 (한양대학교 화학공학과)
이금숙 (한양대학교 화학공학과)
Publication Information
KSBB Journal / v.17, no.3, 2002 , pp. 290-294 More about this Journal
Abstract
The present work describes the improvement of an erythritol-producing strain to lower the formation of glycerol, which is a characteristic by-product of the strain and could cause difficulties in the recovery and purification of the final product. The yeast-like fungi Moniliella suaveolens var. nigra, isolated previously in the same laboratory from beehives, was mutated by exposing it to a 4 g/L NTG solution. From a total of 2000 mutated strains, Em6j30-14 was selected as the one having the most desirable properties. Cultivating the strain for seven days in 300 mL flasks containing 30 mL of a 400 g/L glucose medium resulted in an erythritol yield of 43%. The glycerol yield was 5%, which is a value 50% lower as compared with the wild type. However, attempts to reproduce the above results in a 5L-fermenter failed, resulting in a similar erythritol concentration but a much higher formation of glycerol. Possible reasons for such a different behaviour could be oxygen limitation or the aggregation of cells, but the exact mechanism could not yet be identified. Foam formation, which is another major problem in large-scale fermentation, tended to be much lower for the mutant strain.
Keywords
erythritol; Moniliella suaveolens; fermentation; glycerol;
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