Browse > Article

Isolation of Peptides from Human Blood by RP-HPLC  

Yulia Polyakova (초정밀생물분리기술센터, 인하대학교, 화학공학과)
Publication Information
KSBB Journal / v.17, no.3, 2002 , pp. 261-265 More about this Journal
Abstract
The biologically active peptides in human blood can adjust the functions of many physiological systems. The peptides in human blood were separated on the five steps of linear gradient-elution mode by RP-HPLC with UV detection. The size of commercially available $C_{18}$ chromatographic column was 4.60$\times$150 mm with particle size of 5 $\mu\textrm{m}$ and pore size of 100 $\AA$. The mobile phases used were water in 0.75% trifluoroacetic acids (TFA) and organic modifier of acetonitrile. The isolation methods suggested in this work for peptides from the blood were composed of the formation of immiscible liquid layers and precipitation by centrifuge and chemicals of sodium citrate and trichloroacetic acid (TCA). The some peptides were identified based on the retention times previously constructed database.
Keywords
peptides; Isolation; degradation; RP-HPLC; human blood;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Zhuo, X. Wu, H. Liu, W. Huang, Z (1997), Detennination of basic drugs in blood by RP-HPLC, Fa Yi Xue Za Zhi, 13, 203-204, 253-254
2 Zukowski J., Pawlowska M., Nagatkina M. Annstrong D. W. (1993), High- perfonnance Liquid Chromatographic Enantioseparation of Glycyl dipeptides and tripeptides on Native Cyclodextrin Phases, Mechanistic Considerations, J. Chromo 629, 169-179   DOI   ScienceOn
3 Apffel A. (1995), Peptide mapping, J. Chromo 712, 177-190   DOI   ScienceOn
4 Ebert R. F. (1998), Peptidemapping of fibrogen subunits, J. Chromo 443, 309-316   DOI   ScienceOn
5 Ivanov V. T., Karelin A. A., Philippova M. M., Nazimov I. V., Pletnev V. Z. (1997), Hemoglobin as a source of endogenous bioactive peptides: the concept of a tissuespecific peptide pool, Peptide Sci. 43. 171-188   DOI   ScienceOn
6 Blishchenko E. Yu., Mernenko O. A., Yatskin O. N., Ziganshin R. H., Philippova M. M., Ivanov V. T., Karelin A. A. (1997), Neokyotorphin and neokyotorphin (1-4): secretion by erythrocytes and regulation of tumor cell growth, FEBS Lett. 414, P.125-128   DOI   ScienceOn
7 Florance J., Galdes A., Konteatis Z., Kosarych Z., Langer K., Martucci C. (1987), High Perfonnance Liquid Chromatographic Separation of Peptide and Amino Acid Stereoisomers, J. Chromo 414, 313-323   DOI
8 Mant C. T. and R. S. Hodges. (1991), High-Perfonnance Liquid Chromatography of Peptides and Proteins: Separation, Analysis, and Confonnation, CRC, 938
9 V. Casal, P. J. Martin-Alvarez, T. Harraiz (1996), Comparative prediction of the retention behaviour of small peptides in several Reversed-phase high-perfonnance liquid chromatography columns by using partial least squares and multiple linear regression, Anal. Chimica Acta, 326, 77-84   DOI   ScienceOn
10 Snider L. R., Kirkland J. J. (1979), Introduction to Modem Liquid Chromatography, 2nd ed., Wiley Interscience. N. Y
11 Cantor C. R., Schimmel P. R. (1980), Biophysical chemistry, part I, ch. 2, 5, part 3, ch. 17, 20, 21. W. H. Freeman & Co. San Francisco
12 Seiler P. (1999), Peptide bank regulatory peptides, J. Chromo A. 852, 273-283   DOI   ScienceOn
13 Issaq H. J., (1986), Separation of Selected Dipeptides by High Perfonnance Liquid Chromatography, J. Liq. Chromo 9(1), 229-237   DOI   ScienceOn
14 T. Harraiz (1997), Sample preparation and Reversed-phase high-perfonnance liquid chromatography analysis of foodderived peptides, Anal. Chimica Acta, 352, 119-139   DOI   ScienceOn
15 Pagel, P. Blome, J. Wolf, H U (2000), High-perfonnance liquid chromatographic separation and measurement of various biogenic compounds possibly involved in the pathomechanism of Parkinson's disease, J. Chromo B, 746, 297-304   DOI   ScienceOn
16 A. A. Karelin, M. M. Filippova, O. N. Yatskin, E. Yu. Blishchenko, I. V. Nazimov, and V. T. Ivanov (1998), Proteolytic Degradation of Hemoglobin in Erythrocytes Yields Biologically Active Peptides, Peptide Science, 4, 271-281
17 Chang C. A., Ji H., Lin G. (1990), Effects of Mobile Phase Composition on the Reversed-Phase Separation of Dipeptides and Tripeptides with Cyclodextrin BondedPhase Columns, J. Chromo 522, 143-152   DOI   ScienceOn