Browse > Article

Heavy-Metal Adsorption by Recombinant Saccharomyces cerevisiae Harboring Multiple Copies of the CUP1 Gene  

서진호 (서울대학교 식품공학과 및 농업생물신소재연구센터)
박상옥 (서울대학교 식품공학과 및 농업생물신소재연구센터)
김명동 (서울대학교 식품공학과 및 농업생물신소재연구센터)
한기철 (서울대학교 식품공학과 및 농업생물신소재연구센터)
전영석 (서울대학교 식품공학과 및 농업생물신소재연구센터)
안장우 (청강문화산업대학 건강식품과학과)
한남수 (충북대학교 식품공학과)
Publication Information
KSBB Journal / v.17, no.1, 2002 , pp. 38-43 More about this Journal
Abstract
Characteristics of cell growth and heavymetal adsorption by recombinant Saccharomyus cerevisiae strains harboring multiple copies of the CUP1 gene encoding metallothione (MT) protein were studied in batch cultures. Recombinant S. cerevisiae strains harboring multiple copies of the CUP1 gene were superior to the host and wild-type yeast strains in terms of cell growth and heavy metal removal, indicating that the copy number of the CUP1 gene for MT expression played an important role in the adsorption of heavy metals. It was suggested that the CUP1 promoter for the MT expression is induced by manganese and zinc as well as copper An optimum copper concentration for MT expression and concomitant adsorption of heavy metals by recombinant S. cerevisiae was found to be 0.31 mM. A nonionic surfactant Triton X-100 enhanced cell growth by 17.7% and removal of zinc by 6.1% compared with the control case.
Keywords
metallothionein; heavymetal; CUP1; Saccharomyces cerevisiae;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Microbiological methods for the extraction and recovery of metals /
[ Kelly, D. P.;P. R. Norris;C. L. Brierley ] / Microbial Technology, current state, future prospects
2 The gene for cadmium metallothionein from a cadmium-resistant yeast appears to be identical to CUPI in a copper-resistant strain /
[ Tohoyama, H.;T.Tomoyasu;M. Inoue;M. Joho;T. Murayama ] / Curr. Genet.   DOI   ScienceOn
3 Functionalized monolayers on ordered mesoporous supports /
[ Feng X.;G. E. Fryxell;L.-Q. Wang;A. Y. Kim;J. Liu;K. M. Kemner ] / Science   DOI   ScienceOn
4 Biosorption of heavy metals by Saccharomyces cerevisiae /
[ Volesky, B.;H. A. May-Phillips ] / Appl. Microbiol. Biotechnol.   DOI   ScienceOn
5 Accumulation of metals by microorganisms and algae /
[ Gadd, G. M. ] / Biotechnology- a comprehensive treatise
6 The CUP2 gene product regulates the expression of the CUP1 gene, coding for yeast metallothionein /
[ Welch, J. W.;S. Fogel;C. Buchman;M. Karin ] / EMBO
7 Yeast metallothionein, sequence and metal-binding properties /
[ Dennis, R. W.;K. B. Nielson;R. G. Willianm;D. H. Hamer ] / J. Biol. Chem.
8 Chemical and enzymatic extraction of heavy metal bindings polymers from isolated cell walls of Saccharomyces cerevisiae /
[ Brady, D.;A. D. Stoll ] / Biotech. Bioeng.   DOI   ScienceOn
9 Fungal responses towards heavy metals /
[ Gadd, G. M. ] / microbes in extreme environments
10 Considerations for commercial use of natural products for metals recovery /
[ Brierley, J. A.;G. M. Goyak;C. L. Brierley ] / Immobilization of Ions by Biosorption
11 Multicopy CUP1 plasmids enhance cadmium and copper resistance levels in yeast /
[ Jeyaprakash, A.;J. W. Welch ] / Mol. Gen. Genet.
12 Function and autoregulation of yeast copperthionein /
[ Hamer, D.;D. Thiele;J. Lemontt ] / Science   DOI
13 Growth and copper resistance of recombinant Saccharomyces cerevisiae containing a metalothionein gene /
[ Han, N. S.;J. H. Seo ] / Biotechnol. Lett.   DOI
14 Micoorganism in reclamation of metals /
[ Hurikoshi, T.;M. S. Nakajima;J. A. Brierley;C. L. Brierly ] / Ann. Rev. Microbiol.   DOI   ScienceOn
15 Growth and cadmium removal in recombinant Saccharomyces cerevisiae harboring a metallothionein gene /
[ Kim, D. O.;S. S. Park;J. H. Seo ] / Korean J. Biotechnol. Bioeng.   과학기술학회마을
16 The uptake and cellular distribution of zinc in Saccharomyces cerevisiae /
[ Christopher, W.;M. G. Geoffrey ] / J. General Microbiol.
17 Tandem gene amplification mediates copper-resistance in yeast /
[ Fogel, S.;J. W. Welch ] / Proc. Natl. Acad. Sci. U.S.A.   DOI   ScienceOn
18 Characteristics of growth and metal removal in recombinant Saccharomyces cerevisiae harboring a metallothionein gene /
[ Chung, D. H.;D. O. Kim;J. H. Seo ] / Korean J. Biotechnol. Bioeng.   과학기술학회마을
19 Bioaccumulation of zinc and cadmium in freshwater algae, Chlorella vulgaris, part Ⅰ. toxicity and accumulation /
[ Maeda, S.;M. Mizoguchi ] / Chemosphere   DOI   ScienceOn
20 Biofunctional change in yeast cell surface on treatment with Triton X-100 /
[ Kimura, A.;K. Arima;K. Murata ] / Agric. Biol. Chem.   DOI
21 /
[ Sambook, J.;E. F. Frithsch;T. Maniatis ] / Molecular cloning, a laboratory manual (2nd ed.)
22 Metal resistance and accumulation in bacteria /
[ Belliveau, B. H.;M. E. Starodub;C. Cotter;J. T. Trevors ] / Biotechnol. Adv.   DOI   ScienceOn