Browse > Article
http://dx.doi.org/10.5322/JESI.2022.31.12.1051

Microbial Communities and Diversities in a Full-Scale Mesophilic Anaerobic Digester Treating Sewage Sludge  

Minjae Kim (Division of Earth Environmental System Science (Major of Environmental Engineering), Pukyong National University)
Suin Park (Department of Social Environmental Systems Engineering, Pusan National University)
Juyun Lee (Division of Earth Environmental System Science (Major of Environmental Engineering), Pukyong National University)
Hyebin Lee (Department of Social Environmental Systems Engineering, Pusan National University)
Seonmin Kang (Division of Earth Environmental System Science (Major of Environmental Engineering), Pukyong National University)
Hyokwan Bae (Department of Urban and Environmental Engineering, Ulsan National Institute of Science and Technology)
Joonyeob Lee (Division of Earth Environmental System Science (Major of Environmental Engineering), Pukyong National University)
Publication Information
Journal of Environmental Science International / v.31, no.12, 2022 , pp. 1051-1059 More about this Journal
Abstract
This study investigated microbial communities and their diversity in a full-scale mesophilic anaerobic digester treating sewage sludge. Influent sewage sludge and anaerobic digester samples collected from a wastewater treatment plant in Busan were analyzed using high-throughput sequencing. It was found that the microbial community structure and diversity in the anaerobic digester could be affected by inoculation effect with influent sewage sludge. Nevertheless, distinct microbial communities were identified as the dominant microbial communities in the anaerobic digester. Twelve genera were identified as abundant bacterial communities, which included several groups of syntrophic bacteria communities, such as Candidatus Cloacimonas, Cloacimonadaceae W5, Smithella, which are (potential) syntrophic-propionate-oxidizing bacteria and Mesotoga and Thermovigra, which are (potential) syntrophic-acetate-oxidizing bacteria. Lentimicrobium, the most abundant genus in the anaerobic digester, may contribute to the decomposition of carbohydrates and the production of volatile fatty acids during the anaerobic digestion of sewage sludge. Of the methanogens identified, Methanollinea, Candidatus Methanofastidiosum, Methanospirillum, and Methanoculleus were the dominant hydrogenotrophic methanogens, and Methanosaeta was the dominant aceticlastic methanogens. The findings may be used as a reference for developing microbial indicators to evaluate the process stability and process efficiency of the anaerobic digestion of sewage sludge.
Keywords
Full-scale anaerobic digester; Sewage sludge; Microbial diversity; Microbial communities; High-throughput sequencing;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Ahring, B. K., Sandberg, M., Angelidaki, I., 1995, Volatile fatty acids as indicators of process imbalance in anaerobic digestors. Appl. Microbiol. Biotechnol., 43, 559-565.   DOI
2 Cai, M., Wilkins, D., Chen, J., Ng, S. K., Lu, H., Jia, Y., Lee, P. K., 2016, Metagenomic reconstruction of key anaerobic digestion pathways in municipal sludge and industrial wastewater biogas-producing systems. Front. Microbiol., 7, 778.
3 Callahan, B. J., McMurdie, P. J., Holmes, S. P., 2017, Exact sequence variants should replace operational taxonomic units in marker-gene data analysis. ISME J., 11, 2639-2643.   DOI
4 Carballa, M., Regueiro, L., Lema, J. M., 2015, Microbial management of anaerobic digestion: exploiting the microbiome-functionality nexus. Curr. Opin. Biotechnol., 33, 103-111.   DOI
5 de Bok, F. A. M., Stams, A. J. M., Dijkema, C., Boone, D. R., 2001, Pathway of propionate oxidation by a syntrophic culture of Smithella propionica and Methanospirillum hungatei. Appl. Environ. Microbiol., 67, 1800-1804.   DOI
6 Dyksma, S. Gallert, C., 2019, Candidatus Syntrophosphaera thermopropionivorans: a novel player in syntrophic propionate oxidation during anaerobic digestion. Environ. Microbiol. Rep. 11, 558-570.   DOI
7 Han, G., Shin, S. G., Cho, K., Lee, J., Kim, W., Hwang, S., 2019, Temporal variation in bacterial and methanogenic communities of three full-scale anaerobic digesters treating swine wastewater. Environ. Sci. Pollut. Res. Int., 26, 1217-1226.   DOI
8 Han, G., Shin, S. G., Lee, J., Shin, J., Hwang, S., 2017, A Comparative study on the process efficiencies and microbial community structures of six full-scale wet and semi-dry anaerobic digesters treating food wastes. Bioresour. Technol., 245, 869-875.   DOI
9 Herlemann, D. P. R., Labrenz, M., Jurgens, K., Bertilsson, S., Waniek, J. J., Andersson, A. F., 2011, Transitions in bacterial communities along the 2000 km salinity gradient of the Baltic Sea. ISME J., 5, 1571-1579.   DOI
10 Le, T. N. T., Lee, J., 2021, Effect of ammonia load on microbial communities in mesophilic anaerobic digestion of propionic acid. J. Environ. Sci. Int., 30, 1093-1100.   DOI
11 Lee, J., Kim, E., Han, G., Tongco, J. V., Shin, S. G., Hwang, S., 2018, Microbial communities underpinning mesophilic anaerobic digesters treating food wastewater or sewage sludge: A full-scale study. Bioresour. Technol., 259, 388-397.   DOI
12 Lee, J., Shin, S. G., Han, G., Koo, T., Hwang, S., 2017, Bacteria and archaea communities in full-scale thermophilic and mesophilic anaerobic digesters treating food wastewater: Key process parameters and microbial indicators of process instability. Bioresour. Technol., 245, 689-697.   DOI
13 Riviere, D., Desvignes, V., Pelletier, E., Chaussonnerie, S., Guermazi, S., Weissenbach, J., Li, T., Camacho, P., Sghir, A., 2009, Towards the definition of a core of microorganisms involved in anaerobic digestion of sludge. ISME J., 3, 700-714.   DOI
14 MoE., 2021, Statistics of sewerage, Ministry of Environment, Korea.
15 Nobu, M. K., Narihiro, T., Rinke, C., Kamagata, Y., Tringe, S. G., Woyke, T., Liu, W. T., 2015, Microbial dark matter ecogenomics reveals complex synergistic networks in a methanogenic bioreactor. ISME J., 9, 1710-1722.   DOI
16 Pelletier, E., Kreimeyer, A., Bocs, S., Rouy, Z., Gyapay, G., Chouari, R., Riviere, D., Ganesan, A., Daegelen, P., Sghir, A., Cohen, G. N., Medigue, C., Weissenbach, J., Le Paslier, D., 2008, Candidatus Cloacamonas acidaminovorans: genome sequence reconstruction provides a first glimpse of a new bacterial division. J. Bacteriol., 190, 2572-2579.   DOI
17 Shin, S. G., Han, G., Lee, J., Shin, J., Hwang, S., 2019a, A Snapshot of microbial community structures in 20 different field-scale anaerobic bioreactors treating food waste. J. Environ. Manage., 248, 109297.
18 Shin, J., Cho, S. K., Lee, J., Hwang, K., Chung, J. W., Jang, H. N., Shin, S. G., 2019b, Performance and microbial community dynamics in anaerobic digestion of waste activated sludge: impact of immigration. Energies, 12, 573.
19 Singh, A., Schnurer, A., Westerholm, M., 2021, Enrichment and description of novel bacteria performing syntrophic propionate oxidation at high ammonia level. Environ. Microbiol., 23, 1620-1637.   DOI
20 Spellerberg, I. F., Fedor, P. J., 2003, A Tribute to Claude Shannon (1916-2001) and a plea for more rigorous use of species richness, species diversity and the 'Shannon-Wiener' Index. Glob. Ecol. Biogeogr., 12, 177-179.   DOI
21 Yu, Y., Lee, C., Kim, J., Hwang, S., 2005, Group-specific primer and probe sets to detect methanogenic communities using quantitative real-time polymerase chain reaction. Biotechnol. Bioeng., 89, 670-679.   DOI
22 Sun, L., Toyonaga, M., Ohashi, A., Tourlousse, D. M., Matsuura, N., Meng, X. Y., Tamaki, H., Hanada, S., Cruz, R., Yamaguchi, T., Sekiguchi, Y., 2016, Lentimicrobium saccharophilum gen. nov., sp. nov., a strictly anaerobic bacterium representing a new family in the phylum Bacteroidetes, and proposal of Lentimicrobiaceae fam. nov. Int. J. Syst. Evol. Microbiol., 66, 2635-2642.   DOI
23 Sutcliffe, I. C., 2010, A Phylum level perspective on bacterial cell envelope architecture. Trends Microbiol., 18, 464-470.
24 Tongco, J. V., Kim, S., Oh, B. R., Heo, S. Y., Lee, J., Hwang, S., 2020, Enhancement of hydrolysis and biogas production of primary sludge by use of mixtures of protease and lipase. Biotechnol. Bioprocess Eng., 25, 132-140.   DOI
25 Zhen, G., Lu, X., Kato, H., Zhao, Y., Li, Y. Y., 2017, Overview of pretreatment strategies for enhancing sewage sludge disintegration and subsequent anaerobic digestion: Current advances, full-scale application and future perspectives. Renew. Sust. Energ. Rev., 69, 559-577.   DOI