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http://dx.doi.org/10.5322/JESI.2019.28.2.249

Effects of a Glycoprotein Isolated from Ulmus davidiana Nakai on Toluene-Induced Ecotoxicity and its Mechanism in Human Intestinal Epithelial Cells  

Kim, Do-Wan (Department of Pharmaceutical Engineering, Daegu Haany University)
Kim, Ji-Yun (Department of Pharmaceutical Engineering, Daegu Haany University)
Park, Moon-Ki (Department of Pharmaceutical Engineering, Daegu Haany University)
Lee, Sei-Jung (Department of Pharmaceutical Engineering, Daegu Haany University)
Publication Information
Journal of Environmental Science International / v.28, no.2, 2019 , pp. 249-257 More about this Journal
Abstract
Ulmus davidiana Nakai (UDN) has been traditionally used as a herbal medicine to treat inflammatory diseases in Korea. In the present study, we investigated the anti-ecotoxic potential of a 116 kDa glycoprotein isolated from UDN (UDN glycoprotein) in human intestinal epithelial INT-407 cells. We demonstrated that UDN glycoprotein ($20{\mu}g/mL$) could inhibit the production of lactate dehydrogenase (LDH) induced by toluene, an ecotoxic substance. Additionally, we found that the toluene-induced intestinal cytotoxicity was mediated by the phosphorylation of p38 Mitogen-Activated Protein Kinase (MAPK) via the production of intracellular Reactive Oxygen Species (ROS). The UDN glycoprotein significantly decreased the levels of ROS production and p38 MAPK activation in toluene-stimulated INT-407 cells. Moreover, the UDN glycoprotein inhibits the phosphorylation of nuclear factor-kappa B ($NF-{\kappa}B$), which is responsible for the production of LDH, in toluene-stimulated INT-407 cells. Collectively, our data indicate that UDN glycoprotein is a natural antioxidant and a modulator of ecotoxicity signaling pathways in human intestinal epithelial cells.
Keywords
Ulmus davidiana Nakai (UDN) glycoprotein; Intestinal epithelial cells; Reactive oxygen species; $NF-{\kappa}B$; Toluene;
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