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http://dx.doi.org/10.5322/JESI.2014.5.763

The Electrophysiology Application on Guard Cells to See the Influence of Carbon Dioxide  

Lee, Joon-Sang (The Department of Biology Education, Chungbuk National University)
Publication Information
Journal of Environmental Science International / v.23, no.5, 2014 , pp. 763-770 More about this Journal
Abstract
The effect of $CO_2$ on the opening of stomata in the intact leaf of Commelina communis has been investigated. Full opening of stomatal apertures(around $18{\mu}m$) was achieved in the intact leaf by addition of $CO_2$($900{\mu}mol\;mol^{-1}$). At 90 minutes, the stomatal apertures of leaves treated with $CO_2$ free air were reduced. In contrast, stomata opened most widely with the treatment of $CO_2$ air at 90 minutes. The effects of light, $CO_2$ air and $CO_2$ free air on the change of membrane potential difference(PD) were measured. Fast hyperpolarization of guard cell membrane PD was recorded reaching up to -12 mV in response to light. If $CO_2$ free air was given firstly, there was no response. When light was given after $CO_2$ free air, the light effect was very clear. At the onset of $CO_2$ air, the PD showed a dramatic hyperpolarization to about -25 mV. Changes in the pH of apoplast in intact leaves in response to $CO_2$ air were observed. $CO_2$ air caused a change of 0.4 pH unit. Therefore, it can be hypothesized that $CO_2$ flowing could stimulate proton efflux which is a necessary precursor of stomatal opening.
Keywords
$CO_2$; Commelina communis; Stomatal aperture; Membrane potential difference;
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Times Cited By KSCI : 2  (Citation Analysis)
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