Browse > Article
http://dx.doi.org/10.5322/JES.2010.19.9.1077

Biosynthesis and Control of Keratinase in Recalcitrant Feather-Degrading Bacillus megaterium F7-1  

Jeong, Jin-Ha (Department of Life Science and Environmental Biochemistry, Pusan National University)
Lee, Na-Ri (Department of Life Science and Environmental Biochemistry, Pusan National University)
Jeon, Young-Dong (Department of Life Science and Environmental Biochemistry, Pusan National University)
Kim, Jeong-Do (Department of Life Science and Environmental Biochemistry, Pusan National University)
Park, Ki-Hyun (Department of Life Science and Environmental Biochemistry, Pusan National University)
Park, Geun-Tae (Research and University-Industry Cooperation, Pusan National University)
Son, Hong-Joo (Department of Life Science and Environmental Biochemistry, Pusan National University)
Publication Information
Journal of Environmental Science International / v.19, no.9, 2010 , pp. 1077-1082 More about this Journal
Abstract
This study was performed to investigate the nutritional conditions controlling keratinase activity in Bacillus megaterium F7-1. B. megaterium F7-1 produced keratinase using chicken feather as a sole source of carbon, nitrogen and sulfur. Addition of the feather medium with glucose enhanced keratinase production (68.9 U/ml), compared to control without glucose (63.2 U/ml). The synthesis of keratinase was repressed by addition of $NH_4Cl$ in B. megaterium F7-1. The highest keratinase production (70.9 U/ml) was obtained with the feather medium containing glucose and $MgSO_4{\cdot}7H_2O$. Keratinase was produced in the absence of feather (4.9 U/ml), indicating its constitutive synthesis. Feather degradation resulted in free SH group formation. B. megaterium F7-1 effectively degraded chicken feather meal (86%), whereas duck feather, human nail, human hair and sheep wool displayed relatively low degradation rates (8-34%).
Keywords
Bacillus megaterium; Feather; Keratinase; Biosynthesis control;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Rozs, M., Manczinger, L., Vagvolgyi, Cs., Kevei, F., Hochkoeppler, A., Rodriguez, A. G. V., 2001, Fermentation characteristics and secretion of proteases of a new keratinolytic strain of Bacillus licheniformis, Biotechnol. Lett., 23, 1925-1929.   DOI
2 Sangali, S., Brandelli, A., 2000, Feather keratin hydrolysis by a Vibrio sp. strain kr2, J. Appl. Microbiol., 89, 735-743.   DOI
3 Singh, C. J., 1997, Characterization of an extracellular keratinase of Trichophyton simii and its role in keratin degradation, Mycopathologia, 137, 13-16.   DOI
4 Son, H. J., Kim, Y. G., Park, Y. K., 2004, Isolation and identification of feather-degrading bacteria for biotechnological applications of keratinaceous protein waste, J. Life Sci., 14, 229-234.   DOI
5 Son, H. J., Park, H. C., Kim, H. S., Lee, C. Y., 2008, Nutritional regulation of keratinolytic activity in Bacillus pumilis, Biotechnol. Lett., 30, 461-465.   DOI
6 Wawrzkiewicz, K., Lobarzewski, J., Wolski, T., 1987, Intracellular keratinase of Trichophyton gallinae, J. Med. Vet. Mycol., 25, 261-268.   DOI
7 Joshi, S. G., Tejashwini, M. M., Revati, N., Sridevi, R., Roma, D., 2007, Isolation, identification and characterization of a feather degrading bacterium, Int. J. Poult. Sci., 6, 689-693.   DOI
8 Kumar, A. G., Swarnalatha, S., Gayathri, S., Nagesh, N., Sekaran, G., 2008, Characterization of an alkaline active-thiol forming extracellular serine keratinase by the newly isolated Bacillus pumilis, J. Appl. Microbiol., 104, 411-419.
9 Kunert, J., Stransky, Z., 1988, Thiosulfate production from cysteine by the keratinophilic prokaryote Streptomyces fradiae, Arch. Microbiol., 150, 600-601.   DOI
10 Malviya, H. K., Rajak, R. C., Hasija, S. K., 1992, Synthesis and regulation of extracellular keratinase in three fungi isolated from the grounds of a gelatin factory, Jabalpur, India, Mycophathologia, 120, 1-4.   DOI
11 Noronha, E. F., de Lima, B. D., de Sa, C. M., Felix, C. R., 2002, Heterologous production of Aspergillus fumigatus keratinase in Pichia pastoris, World J. Microbiol. Biotechnol., 18, 563-568.   DOI
12 Onifade, A. A., Al-Sane, N. A., Al-Musallam, A. A., Al-Zarban, S., 1998, Potentials for biotechnological applications of keratin-degrading microorganisms and their enzymes for nutritional improvement of feathers and other keratins as livestock feed resources, Biores. Technol., 66, 1-11.   DOI
13 Park, G. T., Son, H. J., 2009, Keratinolytic activity of Bacillus megaterium F7-1, a feather-degrading mesophilic bacterium, Microbiol. Res., 164, 478-485.   DOI
14 Rao, M. B., Tanksale, A. M., Ghatge, M. S., Deshpande, V. V., 1998, Molecular and biotechnological aspects of microbial proteases, Microbiol. Mol. Biol. Rev., 62, 597-635.
15 Riffel, A., Lucas, F., Heeb, P., Brandelli, A., 2003, Characterization of a new keratinolytic bacterium that completely degrades native feather keratin, Arch. Microbiol., 179, 258-265.
16 Bradford, M. M., 1976, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem., 72, 248-254.   DOI   ScienceOn
17 Brandelli, A., Daroit, D. J., Riffel, A., 2010, Biochemical features of microbial keratinases and their production and applications, Appl. Microbiol. Biotechnol., 85,1735-1750.   DOI
18 Bressollier, P., Letorneau, F., Urdaci, M., Verneuil, B., 1999, Purification and characterization of a keratinolytic serine proteinase from Streptomyces albidoflavus, Appl. Environ. Microbiol., 65, 2570-2576.
19 Ellman, G. L., 1959, Tissue sulfhydryl groups, Arch. Biochem. Biophy., 82, 70-77.   DOI
20 Cheng, S. W., Hu, H. M., Shen, S. W., Takagi, H., Asano, M., Tasi, Y. C., 1995, Production and characterization of keratinase of a feather-degrading Bacillus licheniformis PWD-1, Biosci. Biotechnol. Biochem., 59, 2239-2243.   DOI
21 Fisher, S. H., Sonenshein, A. L., 1991, Control of carbon and nitrogen metabolism in Bacillus subtilis, Annu. Rev. Microbiol., 45, 107-135.   DOI
22 Gioppo, N. M. da R., Moreira-Gasparin, F. G., Costa, A. M., Alexandrino, A. M., de Souza, C. G. M., Peralta. R. M., 2009, Influence of the carbon and nitrogen sources on keratinase production by Myrothecium verrucariain submerged and solid state cultures, J. Ind. Microbiol. Biotechnol., 36, 705-711.   DOI   ScienceOn
23 Haddar, H. O., Zaghloul, T. I., Saeed, H. M., 2009, Biodegradation of native feather keratin by Bacillus subtilis recombinant strains, Biodegradation, 20, 687-694.   DOI
24 Ignatova, Z., Gousterova, A., Spassov, G., Nedkov, P., 1999, Isolation and partial characterization of extracellular keratinase from a wool degrading thermophilic actinomycete strain Thermoactinomyces candidus, Can. J. Microbiol., 45, 217-222.   DOI
25 Bockle, B., Müller, R., 1997, Reduction of disulfide bonds by Streptomyces pactum during growth on chicken feathers, Appl. Environ. Microbiol., 63, 790-792.
26 Atalo, K., Gashe, B. A., 1993, Protease production by a thermophilic Bacillus species (P-001A) which degrades various of fibrous proteins, Biotechnol. Lett., 15, 1151-1156.   DOI