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http://dx.doi.org/10.5322/JES.2008.17.2.163

Protoplast Fusion of Panax ginseng Callus and Aralia Continentalis Mesophyll  

Park, Jong-Bum (Department of Biological Sciences, Silla University)
Publication Information
Journal of Environmental Science International / v.17, no.2, 2008 , pp. 163-170 More about this Journal
Abstract
Protoplasts of Panax ginseng C. A. Meyer and Aralia continentalis K. (Araliaceae) were isolated from callus cells and mesophyll cells, respectively. The maximum yield of protoplasts isolated from callus cells of P. ginseng were obtained by incubation for 3 hrs in the enzyme mixture of 0.5% macerozyme, 1.5% cellulase, and 0.5 M mannitol as an osmoticum. In the case of mesophyll cells of A. continentalis, the highest yield of protoplasts were obtained by incubation for 5 hrs in the enzyme mixture of 1% macerozyme, 2% cellulase, and 0.6 M mannitol. A polyethylene glycol (PEG) treatment induced an intergeneric fusion of the protoplasts. The fusion products, that is, heterokaryocytes were obtained by treatment of 50% PEG containing 0.05 M Ca salts.
Keywords
Panax ginseng; Aralia continentalis; Protoplast; Fusion; PEG; Heterokaryocyte;
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1 Takebe I., Nagata T., 1984, Isolation and culture of protoplasts: Tobacco. In Cell culture and somatic cell genetics of plants, Vasil, I. K. (ed.), Academic Press Inc. London, 328-339pp
2 Chatterjee G., Sikder S. R., Sen S. K., 1985, Regeneration of plantlets from mesophyll protoplasts of Brassica juncea (L.) Czern., Plant Cell Reports, 4, 245-247   DOI   ScienceOn
3 Kamlesh R. P., Shekhawat N. S., Berlyn G. P., Thorpe T. A., 1984, Isolation and culture of protoplasts from cotyledons of Pinus coulteri Don., Plant Cell Tissue Organ Culture, 3, 85-90   DOI
4 Ruesink A. W., Thimann K. V., 1965, Protoplasts from the Avena coleoptile, Proc. Nat. Acad. Sci. U.S.A., 54, 56-64
5 Barakat M. N., Cocking E. C., 1985, An assessment of the cultural capabilities of protoplast of some wild species of Linum, Plant Cell Reports, 4, 164-167   DOI   ScienceOn
6 Du L., Bao M., 2005, Plant regeneration from protoplasts isolated from embryogenic suspension cultured cells of Cinnamomum camphora L., Plant Cell Reports, 24, 462-467   DOI
7 Keller W. A., Melchers G., 1973, The effect of high pH and calcium on tobacco leaf protoplast fusion, Z. Naturforsch., 280, 737-741
8 Kao K. N., Michayluk M. R., 1974, A method for high-frequency intergeneric fusion of plant protoplasts, Planta, 115, 355-367   DOI   ScienceOn
9 Uchimiya H., Murashige T., 1974, Evaluation of parameters in the isolation of viable protoplasts from cultured tobacco cells, Plant Physiol., 54, 936-944.   DOI   ScienceOn
10 Schenk R. U., Hildebrandt. A. C., 1969, Production of protoplasts from plant cells in liquid culture using purified commercial cellulase, Crop Sci., 9, 629-631   DOI
11 Hou S. W., Jia J. F., 2004, Plant regeneration from protoplasts isolated from embryogenic calli of the forage legume Astragalus melilotoides Pall., Plant Cell Reports, 22, 741-746   DOI
12 Jiang S. H., Guan R. Z., Tang S. Y., Xin R. Y., Zhang H. S., Zhao L. Q., Pan Q. Y., 2007, Somatic hybrids between Rorippa indica (Linn.) Hiern and Brassica napus L. through protoplast-fusion system, Yi Chuan, 29, 745-750   DOI   ScienceOn
13 Brisa M. C., Segura J., 1987, Isolation, culture and plant regeneration from mesophyll protoplasts of Digitalis obscura, Physiol. Planta., 69, 680-686   DOI
14 Szabados L., Gaggero C., 1985, Callus formation from protoplasts of a sugarbeet cell suspension culture, Plant Cell Reports, 4, 195-198   DOI   ScienceOn
15 Wang Y. P., Sonntag K., Rudloff E., 2003, Development of rapeseed with high erucic acid content by asymmetric somatic hybridization between Brassica napus and Crambe abyssinica, Theor. Appl. Genet., 106, 1147-1155   DOI
16 Gupta P. K., Durzan D. J., 1986, Isolation and cell regeneration of protoplasts from sugar pine, Plant Cell Reports, 5, 346-348   DOI   ScienceOn
17 Murashige T., Skoog F., 1962, A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol. Plant., 15, 473-497   DOI
18 Park J. B., 1998, An electron microscopic study on the cell wall regeneration of cultured Panax ginseng callus protoplast, Kor. J. Plant Tissue Culture, 25, 495-500   과학기술학회마을
19 Dorion N., Chupeau Y., Bourgin J. P., 1975, Isolation, culture and regeneration into plants of Ranunculus sceleratus L. leaf protoplasts, Plant Sci. Lett., 5, 325-331   DOI   ScienceOn
20 Kohlenbach H. W., Bohnke E., 1975, Isolation and culture of mesophyll protoplasts of Hyoscyamus niger L. var. annuus Sims., Experientia, 34, 1281-1283
21 Saxena P. K., Gill R., Rashid A., Maheshwari S. C., 1981, Isolation and culture of protoplasts of Capsicum annuum L., Protoplasma, 103, 357-361
22 Binding H., Nehls R., Kock R., Finger J., Mordhorst G., 1981, Comparative studies on protoplast regeneration in herbaceous species of the Dicotyledoneae class, Z. Pflanzenphysiol., 101, 119-130   DOI
23 Power J. B., Frearson E. M., George D., Evans P. K., Berry S. F., Hayward C., Cocking E. C., 1976, Isolation, culture and regeneration of leaf protoplasts in the genus Petunia, Plant Sci. Lett., 7, 51-55   DOI   ScienceOn
24 Mii M., Seeni S., Fowke L. C., King J., 1985, The isolation and cultivation of protoplasts from cell suspensions of a pantothenote-requiring auxotroph of Datura, Can. J. Bot., 63, 779-783   DOI
25 Melchers G., Sacristan M. D., Holder A. A., 1978, Somatic hybrid plants of potato and tomato regenerated from fused protoplasts, Carlsberg Res. Commun., 43, 203-218   DOI
26 Flick C. E., Evans D. A., 1983, Isolation, Culture, and plant regeneration from protoplasts isolated from flower petals of ornamental Nicotiana species, Z. Pflanzenphysiol., 109, 379-382   DOI
27 Liu F., Ryschka U., Marthe F., Kloche E., Schumann G., Zhao H., 2007, Culture and fusion of pollen protoplasts of Brassica oleracea L. var. italica with haploid mesophyll protoplasts of B. rapa L. ssp., Protoplasma, 231, 89-97   DOI
28 Durieu P., Ochatt S. J., 2000, Efficient intergeneric fusion of pea (Pisum sativum L.) and grass pea (Lathyrus sativus L.) protoplasts, J. Exp. Bot., 51, 1237-1242   DOI   ScienceOn
29 Grambow H. J., Kao K. N., Miller R. A., Gamborg O. L., 1972, Cell division and plant development from protoplasts of carrot cell suspension culture, Planta, 103, 348-355   DOI   ScienceOn
30 Cocking E. C., 1960, A method for the isolation of plant protoplasts and vacuoles, Nature, 187, 962-963   DOI
31 Horine R. K., Ruesink A. W., 1972, Cell wall regeneration around protoplasts isolated from Convolvulus tissue culture, Plant Physiol., 50, 438-445   DOI   ScienceOn
32 Shepard J. E., Totten R. E., 1975, Isolation and regeneration of tobacco mesophyll cell protoplasts under low osmotic conditions, Plant Physiol., 55, 689-694   DOI   ScienceOn
33 Evans D. A., Flick C. E., 1980, Somatic hybrid plants between species of the genus Nicotiana, In vitro, 16, 217
34 Gleba Y. Y., Monot V. P., Cherep N. N., Skarzynskaya M. V., 1982, Intertribal hybrid cell lines of Atropa belladonna (x) Nicotiana chinensis obtained by cloning individual protoplast fusion products, Theor. Appl. Genet., 62, 75-79
35 Vardi A., Speigel-Roy P., Galun E., 1982, Plant regeneration from Citrus protoplasts: Variability in methodological requirements among cultivars and species, Theor. Appl. Genet., 62, 171-176   DOI   ScienceOn
36 Gresshoff P. M., 1980, In vitro culture of white clover, callus, suspension, protoplast culture, and plant regeneration, Bot. Gaz., 141, 157-164   DOI   ScienceOn
37 Yamagishi H., Landgren M., Forsberg J., Glimelius K., 2002, Production of asymmetric hybrids between Arabidopsis thaliana and Brassica napus utilizing an efficient protoplast culture system, Theor. Appl. Genet., 104, 959-964   DOI
38 Nelson R. S., Creissen G. P., Bright S. W. J., 1983, Plant regeneration from protoplasts of Solanum brevidens, Plant Sci. Lett., 30, 355-362   DOI   ScienceOn
39 Gosch G., Bajaj Y. P. S., Reinert J., 1975, Isolation, culture and induction of embryogenesis in protoplasts from cell suspensions of Atropa belladonna, Protoplasma, 86, 405-410   DOI
40 Bui-Dang-Ha D., Mackenzie I. A., 1973, The division of protoplasts from Asparagus officinalis L. and their growth and differentiation, Protoplasma, 78, 215- 221   DOI
41 Vardi A., Hutchison D. J., Galun E., 1986, A protoplast-to-tree system in Microcitrus based on protoplasts derived from a sustained embryogenic callus, Plant Cell Reports, 5, 412-414   DOI   ScienceOn
42 Robertson D., Earle E. D., 1986, Plant regeneration from leaf protoplasts of Brassica oleracea var. italica cv. Green Comet broccoli, Plant Cell Reports, 5, 61-64   DOI   ScienceOn
43 Nishimaki T., Nozue M., 1985, Isolation and culture of protoplasts from high anthocyanin-producing callus of sweet potato, Plant Cell Reports, 4, 248-251   DOI   ScienceOn
44 Hu Q., Hansen N., Laursen J., Dixelius C., Andersen B., 2002, Intergeneric hybrids between Brassica napus and Orychophragmus violaceus containing traits of agronomic importance for oilseed rape breeding, Theor. Appl. Genet., 105, 834-840   DOI