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http://dx.doi.org/10.5322/JES.2005.14.7.649

Strain Identification and Comparative Analysis of Toxigenic Cyanobacteria Determined by PCR  

Jung Jong-Mun (Busan Water Quality Institute, Water Authority)
Jung Eun-Young (Busan Water Quality Institute, Water Authority)
LEE You-Jung (Busan Water Quality Institute, Water Authority)
Park Hong-Ki (Busan Water Quality Institute, Water Authority)
Jung Mi-Eun (Busan Water Quality Institute, Water Authority)
Ji Ki-Won (Busan Water Quality Institute, Water Authority)
Joo Gea-Jae (Department of Biology, Pusan National University)
Publication Information
Journal of Environmental Science International / v.14, no.7, 2005 , pp. 649-655 More about this Journal
Abstract
Microcystis aeruginosa is common form of cyanobacteria (blue-green algae) capable of producing toxic heptapeptide (microcystin) that cause illness or death. The comparison of molecular genetic method with the morphological characteristics of cyanobacteria was conducted. We have designed PCR primers (JJM98F, JJM1141R) for cyanobacterial 16S rRNA and phycocyanin intergenic spacer (PC-IGS) gene domain. To confirm the production of microcystins, PCR primers for the N-methyltransferase (NMT) domain of microcystin synthetase gene mcyA were designed using 21 cyanobacteria strains Most of isolated strains from the Nakdong River was classified as Microcystis aeruginosa and the similarities were $99\%$ with M. aeruginosa AF 139292. $38.1\%$ of isolated strains contained microcystin synthesis gene. NMT (N-methyltransferase) were not detected in isolated strain in several strains, which means non-toxic. However, the NMTs of the strains were detected during the cultivation.
Keywords
Cyanobacteria; Microcystins; 16S rRNA; N-methyltransferase (NMT) domain;
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