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http://dx.doi.org/10.5322/JES.2002.11.9.971

Characterization of Biodegradation of Highly Concentrated Phenol by Rhodococcus sp. EL-GT  

Park, Geun-Tae (Department of Microbiology, Pusan National University)
Cha, Mi-Sun (Department of Microbiology, Pusan National University)
Nam, Gui-Sook (Department of Microbiology, Pusan National University)
Cho, Sun-Ja (Department of Microbiology, Pusan National University)
Son, Hong-Joo (Department of Biotechnology, Miryang National University)
Lee, Geon (Department of Microbiology, Pusan National University)
Lee, Sang-Joon (Department of Microbiology, Pusan National University)
Publication Information
Journal of Environmental Science International / v.11, no.9, 2002 , pp. 971-977 More about this Journal
Abstract
The isolated strain, Rhodococcus sp. EL-GT was able to degrade high phenol concentrations up to 10 mM within 24 hours in the medium consisting of 5.3 mM $KH_2PO_4$. 95 mM $Na_2HPO_4$, 18mM $NH_4NO_3$, 1 mM $MgSO_4{\cdot}7H_2O$,\;50{\mu}M CaCl_2$,\;0.5 {\mu}M FeCl_3$, initial pH 8.0, temperature $30^{\circ}C$ in rotary shaker at 200 rpm. This strain was good cell growth and phenol degradation in the alkaline pH range range, and the highest in the pH range of 7 to 9. The microorganism was able to grow at the various chlorinated phenols, benzene, toluene, and bunker-C oil. As Rhodococcus sp. EL-GT was good capable of attachment on the acryl media, it would be used as microorganism to consist of biofilm in wastewater treatment.
Keywords
Rhodococcus sp.; sludge; RBC; biofilm reactor;
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